Purpose Breast cancer has a high prevalence in Korea. To achieve personalized therapy for breast cancer, long-term follow-up specimens are needed for next-generation sequencing (NGS) and multigene analysis. Formalin-fixed paraffin-embedded (FFPE) samples are easier to store than fresh frozen (FF) samples. The objective of this study was to optimize RNA extraction from FFPE blocks for NGS. Met...

Background and Aims: HDV is a defective satellite virus and classified in genus Deltavirus. Its disease is related and limited to HBV-infected patients. Acute infection of delta agent occurs in two different patterns simultaneous infection with both HBV & HDV or super infection of chronically HBV infected patients that lead to more sever type of hepatitis. According to genetic diversity of geno...

Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...

background: angiogenesis, which is required for embryonic development and many physiological events, plays crucial role in many pathological conditions such as tumor growth and metastasis. recent studies indicate anticancer and antitumor properties of saffron against human cancers. many processes are affected by electromagnetic field (emf) and its effect on proliferation and gene expression wer...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

BACKGROUND The study was aimed to show the effect of molecular mechanism of Aqueous Garlic Extract (AGE) on expression of IFNγ and iNOS genes in Leishmania major. METHODS Leishmania major promastigotes (MRHO/IR/75/ER) were added to the in-vitro cultured J774 cell line, the cells were incubated for 72 hours. Various concentrations of garlic extract (9.25, 18.5, 37, 74, 148 mg/ml) were added to...

Introduction Quantitation of RNA samples is necessary for a number of different molecular biology techniques. These include measuring yields of synthetic ribonucleotides or in vitro transcribed RNAs, determining RNA concentrations prior to Northern blot analysis, S1 nuclease or RNase protection assays, cDNA library creation, as well as reverse transcriptase PCR (RT-PCR), and differential displa...