An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lipase has an apparent molecular mass of 67 kDa. The optimum temperature and pH for the purified lip...

background: pain and limited range of motion (rom) are the crucial subsequent results of joint hemorrhages in individuals with bleeding disorders and hemophilia. exercise interventions are particularly recommended in treatment of such patients. the purpose of this study was to detect the influences of conventional exercise therapy and hydrotherapy on the knee joint complications in patients wit...

Sea buckthorn oil (SBO) contains fatty acids that have an anti-aging effect. The delivery system ofSBO can be improved by a nanoemulsion system. purpose of this study was to determine thephysical stability SBO (NSB) with Tween 80 variation. NSB made 1% SBOand variation 35% (FI); 40% (FII) and 45% (FIII). stored in climatic chamberat 30 ± 2°C RH 65%±5% for 90 days. Samples were taken before stor...

alkaline protease enzyme produced from bacillus licheniformis ptcc1331 in optimum condition(temperature 37 ?c, 150 rpm, ph=10), is immobilized in different concentrations of sodium alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. maximum enzymatic activity of 46 was obtained in 2%w/v sodium alginate solution. investigat...

NADP+-linked isocitrate dehydrogenase (E.C.1.1.1.42) has been purified to homogeneity from germinating pea seeds. The enzyme is a tetrameric protein (mol wt, about 146,000) made up of apparently identical monomers (subunit mol wt, about 36,000). Thermal inactivation of purified enzyme at 45 degrees and 50 degrees C shows simple first order kinetics. The enzyme shows optimum activity at pH range...

In fetal sheep, the electrocorticogram (ECOG) recorded directly from the cortex during repetitive heart rate (FHR) decelerations induced by umbilical cord occlusions (UCO) predictably correlates with worsening hypoxic-acidemia. In human fetal monitoring during labor, the equivalent electroencephalogram (EEG) can be recorded noninvasively from the scalp. We tested the hypothesis that combined fe...

A sensitive method is presented for the spectrophotometric determination of phenothiazine derivatives of biological importance. The drugs namely, chlorpromazine hydrochloride (CPH), promethazine hydrochloride (PH), trifluoperazine hydrochloride (TFPH), prochlorperazine maleate (PCPM) and fluphenazine hydrochloride (FPH) are reacted with a fixed amount of dichromate in acidic conditions. After t...