the κ-casein protein plays a fundamental role in milk production and its composition synthesis in the mammary gland. this protein has an important role in the formation, stabilization, and aggregation of the casein micelles. in this study, we investigated the genetic variability at the exon 4 of the κ-casein gene (also known as csn3) using pcr-sscp analysis and dna sequencing. then a protein se...

six red sokoto (rs) and west african dwarf (wad) does, raised intensively in livestock farm of michael okpara university were evaluated for milk yield and composition in a ten-week study. the animals in their first parity were fed cut-and-carry forages in a cafeteria arrangement between 07:00-08:00 h daily and also were allowed access to concentrate supplementation by 14:00 h. the forage consis...

Lactobacillus brevis accumulates lactose and nonmetabolizable lactose analogues via sugar/H+ symport, but addition of glucose to the extracellular medium results in rapid efflux of the free sugar from the cells due to the uncoupling of sugar transport from proton transport. By using vesicles of L. brevis cells, we recently showed that these regulatory/effects could be attributed to the metaboli...

The objective of this study was to evaluate some compositional and physicochemical changes that occur during UHT (Ultra-high temperature) milk storage for up four months. A total 120 samples were collected from two dairy plants in the state Minas Gerais, Brazil, stored under temperatures (20°C 30°C) five periods (0, 30, 60, 90 days). experimental design completely randomized, a 2x2x5 factorial ...

We used preS2-S'-beta-galactosidase, a three-domain fusion protein that aggregates extensively at 43 degrees C in the cytoplasm of Escherichia coli, to search for multicopy suppressors of protein aggregation and inclusion body formation and took advantage of the known differential solubility of preS2-S'-beta-galactosidase at 37 and 43 degrees C to develop a selection procedure for the gene prod...

The objectives of this study were to determine carbohydrates such as glucose, total protein, lipid parameters, mineral levels in blood; qualitative characteristics fat, SNF, lactose, solids and protein percentages milk; the quantity milk after feeding german maize fodders. Thirty clinically apparently healthy cows selected divided into three homogenous groups. Control group was fed mixed grass,...

BACKGROUND The relationship between lactose-maldigestion, self-reported milk intolerance and gastrointestinal symptoms has not been clearly defined. OBJECTIVES To evaluate: a) the prevalence of lactose maldigestion and lactose intolerance in a sample of the general population taken from a rural center; b) the frequency of self-reported milk-intolerance and its correlation with lactose-maldige...

Transcriptional regulatory networks allow bacteria to express proteins only when they are needed. Adaptive hypotheses explaining the evolution of regulatory networks assume that unneeded expression is costly and therefore decreases fitness, but the proximate cause of this cost is not clear. We show that the cost in fitness to Escherichia coli strains constitutively expressing the lactose operon...