BACKGROUND: A major issue in many gene expressionstudies utilizing small amount of biological materials is thelimited quantity of RNApurified from clinical samples, which isoften used for RT-PCR or standard Northern blot analysis.OBJECTIVES: The SMART cDNA synthesis method and subsequentSMART-cDNA-PCR technique was used to analyse 3genes in macroschizonts of Theileria annulata in small lymphnod...

Toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. Toxoplasma gondii-derived heat shock protein 70 (Hsp70) may play an important role in the virulence of Toxoplasma gondii (T. gondii). In the present study, T. gondii Hsp70 was amplified by polymerase chain reaction (PCR) from the DNA of the T. gondiitachyzoite RH strain through the use o...

Brucella bacterium causes Brucellosis, an infectious disease spreading from animals to human. Brucella lumazine synthase (BLS) is a highly immunogenic protein with adjuvant properties, which has been introduced as an effective protein carrier for vaccine development. This protein also plays a significant role in inducing immune system. This study aimed to clone, express, and purify the BLS gene...

Effective identification and understanding of genes contribute to improve plant drought resistance. A study was conducted to identify drought responsive candidate genes in sugarcane. Two genes viz., SOD (Superoxide dismutase) and IGS (Indole 3glycerol phosphate synthase) were used as gene specific markers. Specific primers were designed based on the sequences in Genbank databases. Mapping popul...

Aeromonas hydrophila is one of the serious bacterial pathogen in fresh water aquaculture industry. Recently, some attention has been given to bacterial outer membrane protein (BOMP) as potentially important vaccine components. Ornamental gold fish Carassius auratus immunized with the bacterial outer membrane protein encoding gene clone, BOMPG-pTZ57R/T that was named as BOMPG and the bacterial o...

BACKGROUND Alternative splicing is an important mechanism that regulates gene expression and function in human cells. OCT4, a crucial pluripotency marker in embryonic stem/carcinoma cells generates several spliced variants in different cell types and cancers. The expression of OCT4 in cancers has been challenged in many studies. The existence of several OCT4 spliced variants and absence of spec...

BACKGROUND Adhesion protein E (PE) of Haemophilus influenzae is a 16 - 18 kDa protein with 160 amino acids which causes adhesion to epithelial cells and acts as a major factor in pathogenesis. OBJECTIVES In this study, we performed cloning, expression and purification of PE as a candidate antigen for vaccine design upon further study. MATERIALS AND METHODS At first, the pe gene of NTHi ATCC...