Objective: A pilot study to test the hypothesis that dysregulated expression of haemostasis genes in clonal granulocytes of MPD patients contributes to the genesis of haemostatic complications. In this study of 36 patients with MPD and 18 healthy controls, specific target genes Procoagulant Tissue Factor [TF], prothrombotic cytokines Interlukin1 β [IL1β],Tumor Necrosis Factor α [TNFα],Vascular ...

We analyzed the performance characteristics of the qualitative AMPLICOR CMV Test (Roche Molecular Systems, Pleasanton, Calif.) and quantitative COBAS AMPLICOR CMV MONITOR Test (Roche Molecular Systems) assays and compared the performance of the AMPLICOR quantitative assay with an in-house-developed cytomegalovirus (CMV) DNA PCR assay. The quantitative AMPLICOR assay was found to be more sensiti...

The traditional cultural method (PCR and Real-Time PCR) for Salmonella spp. detection identification is laborious time-consuming. A qualitative LAMP detecting was validated in compliance with ISO 16140:2016. results show a relative accuracy, sensitivity, specificity of 100% comparison the reference 6579-1:2017; LOD50 set as 0.4 CFU/g. Additionally, field study carried out comparing kit, commerc...

BACKGROUND Laboratory diagnostic techniques able to detect Mycoplasma agalactiae are essential in contagious agalactia in dairy goats. This study was designed: 1) to determine the detection limits of PCR and culture in goat milk samples, 2) to examine the effects of experimental conditions including the DNA extraction method, PCR technique and storage conditions (fresh versus frozen stored milk...

Proper selection of endogenous reference genes and their real-time PCR assays is quite important in genetically modified organisms (GMOs) detection. To find a suitable endogenous reference gene and its real-time PCR assay for common wheat (Triticum aestivum L.) DNA content or copy number quantification, four previously reported wheat endogenous reference genes and their real-time PCR assays wer...

The goal of this study was to evaluate serial cytomegalovirus (CMV) blood culture, antigenemia testing, and qualitative and quantitative plasma CMV PCR for their ability to predict CMV disease and thus to direct preemptive therapy after lung transplantation. Forty-one patients provided 414 samples for blood culture, 290 samples for antigenemia testing, and 432 samples for PCR. Seven patients de...

Reverse transcription PCR (RT-PCR) is prone to false positives when contaminating DNA molecules are present at the start of a reaction. Contaminants that derive from earlier work using a given primer pair (carryover PCR products) are of particular concern when those primers are used routinely, as in clinical diagnostics or environmental monitoring. In addition, contamination by genomic DNA can ...