The aim of this study was to develop an event-specific qualitative and real-time quantitative polymerase chain reaction (PCR) method for detection of herbicide-tolerance genetically modified (GM) soybean A2704-12. The event-specific PCR primers were designed, based on the 5'-flanking integration sequence in the soybean genome, to amplify the 239-bp target fragment. Employing the same event-spec...

AIM To identify cytomegalovirus (CMV) infection in liver transplant recipients by polymerase chain reaction (PCR) techniques and to separate the cases in which CMV related disease will occur, for whom treatment is indicated, from those in whom infection will remain innocuous. METHODS The combination of qualitative and semiquantitative PCR of serum and urine was assessed to determine whether t...

We read the Article by Lescure and colleagues1Lescure FX Bouadma L Nguyen D et al.Clinical virological data of first cases COVID-19 in Europe: a case series.Lancet Infect Dis. 2020; (published online March 27.)https://doi.org/10.1016/S1473-3099(20)30200-0Summary Full Text PDF PubMed Scopus (746) Google Scholar with great interest. During ongoing coronavirus disease 2019 (COVID-19) pandemic, mon...

objectives: human cytomegalovirus (cmv) is a major life-threatening pathogen for hematopoietic stem cell transplant recipients. specific tests are used for the diagnosis and monitoring of cmv infection in transplant patients. this study evaluates the performance of pp65 antigenemia and qualitative pcr assays for monitoring cmv in such patients. methods: we analyzed 179 clinical samples from 41...

Follicular lymphoma (FL) is characterized by the t(14;18)(q32;q21) chromosomal translocation which can be detected by polymerase chain reaction (PCR) in approximately 70% of cases. The aim of our retrospective study was to evaluate the sensitivity and the reproducibility of both conventional qualitative and quantitative PCR assays for detection of the t(14;18)(q32;q21) chromosomal translocation...

Abstract The world is facing an exceptional pandemic caused by SARS-CoV-2. To allow the diagnosis of COVID-19 infections, several assays based on real-time PCR technique have been proposed. requests for are such that it was immediately clear choice most suitable method each microbiology laboratory had to be based, one hand, availability materials, and other personnel training priorities this ac...

Urine and blood leukocyte cultures and qualitative plasma polymerase chain reaction (PCR) and quantitative competitive (QC) PCR were evaluated for their ability to identify AIDS patients at risk for human cytomegalovirus (HCMV) disease. AIDS patients were followed with urine and blood specimens every 3 months. During a mean follow-up of 12 months, 26 (28%) developed HCMV disease. The sensitivit...