The sequence analysis of the 5' ends of cDNAs prepared using the anchor ligation method has revealed that most of the full-length cDNAs have an additional dGMP at their 5' end that is absent in the corresponding genome sequence. Using model RNA transcripts with cap analogues possessing 7-methylguanosine and adenosine, the base of the added nucleotide has been shown to be complementary to the ba...

The bidentate RNase III Dicer cleaves microRNA precursors to generate the 21-23 nt long mature RNAs. These precursors are 60-80 nt long, they fold into a characteristic stem-loop structure and they are generated by an unknown mechanism. To gain insights into the biogenesis of microRNAs, we have characterized the precise 5' and 3' ends of the let-7 precursors in human cells. We show that they ha...

BACKGROUND Studies focusing on next-generation sequencing of the bacterial 16S rRNA gene have allowed detailed surveys of skin bacterial populations (microbiota) of the skin. HYPOTHESIS/OBJECTIVES This study evaluated temporal changes in the skin microbiota in a canine model of atopic dermatitis. ANIMALS Eight atopic dogs previously sensitized with house dust mites (HDM). METHODS The dogs...

purpose: to evaluate visual outcomes and intraocular pressure (iop) after single-site phacotrabeculectomy with mitomycin c in patients with senile cataract and primary open angle glaucoma (poag), pseudoexfoliative glaucoma (pxg), or chronic angle closure glaucoma (cacg). methods: in this retrospective study, patients older than 50 years who had significant cataracts (bcva £ 20/60) and uncontrol...

Background: Endometriosis is one of the most common benign gynecological diseases which is characterized by endometriallike tissue growing outside the uterine cavity. Although the pathology of endometriosis remains unknown, the genetic predisposition plays an apparent role. Several genes have been contributed to endometriosis, but it seems KRAS has a crucial role, because its activation results...

Estimating the on-site and off-site impacts of soil erosion as a function land use climate conditions in semi-arid basins is key for water conservation strategies. However, research gap exists theme, requiring further investigation using local hydrological data. To accomplish it, SDR-InVEST model was applied to Pardo-FB basin (Brazil) different use, conservation, conditions. The mean annual los...