It has been known for decades that microbial fermentation within the rumen is critical to postnatal rumen epithelial growth and maturation in ruminants, but the underlying mechanism is largely unknown. In this study, we determined the effect of rumen fluid, which should contain all products from rumen fermentation, on growth of rumen epithelial cells in vitro. Addition of 10% rumen fluid from c...

background: the aim of the present study was to evaluate the efficacy of autologous serum eye drop in treatment of recurrent corneal epithelial defect. m aterials and methods: fourteen patients with recurrent corneal epithelial defect were studied. autologous serum was prepared from the patients and diluted in 20% normal saline. the patients were instructed to use the autologous serum every six...

Assessment of the rumen fluid is an ancillary examination technique used in the clinical examination both of the individual bovine patient and at population level. The specific function and structure of the rumen makes ruminants different from other animals. Therefore, it logical to assess the rumen in detail when confronted with a bovine patient. Assessment of the rumen fluid is essential to a...

Little information is available on the enzyme activities of sheep tissues in vitro. Kleiber (1941, 1947) measured the oxygen consumption of liver slices of rats, rabbits and sheep, and attempted to correlate the QO, of this tissue with the metabolic rate of the living animal and to establish a direct relationship to body size. However, Krebs (1950), using more refined and standardized technique...

The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found i...