nutritive value of rumen epithelial scraping (rep) was assessed using in sacco and in vivo procedures. concentrates were so formulated that 0% (a), 35% (b), 70% (c), and 100% (d) of soybean meal (sbm), were replaced (weight for weight) with rep. in a completely randomized design, 16 west african dwarf (wad) goats were randomly allocated to the experimental diets to assess feed intake and digest...

In order to elucidate the role of propionate in themetabolism of acetate and butyrate in rumen epithelium, the effects of propionate addition on the "C transfer from "C-acetate and -butyrate into CO2, glucose, ketone bodies and lipids fractions in rumen epithelium of normally fed, starved and alloxan diabetic sheepwere investigated. The experimental results are summarized as follows.1) In rumen...

1. [3H]leucine infused directly into the rumen of sheep labelled microbial protein and, when compared with the specific activity of the rumen free-leucine pool, indicated that 50% of the bacterial protein leucine originated from the rumen free-leucine pool. 2. The lower limit for bacterial protein turnover in the rumen was 0.37/d when calculated as the difference between the specific rate of di...

Rumen epithelium is a parakeratotic, stratified squamous epithelium which functions in metabolism, absorption, and protection . In previous light microscope histochemical studies (1, 3) and electron microscope studies (2) on this tissue, phenomena characteristic for both mucus-producing and keratinizing epithelia were described . The presence of mucus was demonstrated histochemically (1, 3) by ...

Emerging evidence at the mRNA level indicates that feeding high-grain diets to ruminants leads to coordinated changes in the molecular response of the rumen epithelium. Yet, epithelial adaptation of the hindgut to increasing dietary grain levels has not been established in ruminants. Therefore, the objective of this study was to characterize alterations in mRNA expression associated with nutrie...

Short-chain fatty acids (SCFAs), such as butyrate, produced by gut microorganisms, play a critical role in energy metabolism and physiology of ruminants as well as in human health. In this study, the temporal effect of elevated butyrate concentrations on the transcriptome of the rumen epithelium was quantified via serial biopsy sampling using RNA-seq technology. The mean number of genes transcr...

It was reported that there was a relationship between the acetate metabolism and TCA cycle in rumen epithelium of sheep. The present study was designated to elucidate how this relationship was influenced by starvation and alloxan treatment. The results obtained are summarized as follows. There was no effect of the addition of citrate or succinate on the "C transfer from "C-acetate into CO„gluco...

It was reported that there was a relationship between the propionate metabolism and TCA cycle, and that starvation and alloxan treatment influenced the propionate metabolism, and the relationships between the propionate metabolism and glucolysis were changed by the alloxan treatment in the rumen epithelium of sheep. Further study was designed to elucidiate the relationship betweenthe propionate...

Bacteria attached to the rumen epithelium (or epimural community) are not well characterised and their role in rumen functioning is not totally understood. There is just one published report of a clone library from one cow that suggests that this epimural community differs from the bacteria associated with the rumen digestive contents. However, this time-consuming approach is not adapted for ex...

Currently, the mechanism(s) responsible for the regulation of urea transporter B (UT-B) expression levels in the epithelium of the rumen remain unclear. We hypothesized that rumen fermentation products affect ruminal UT-B expression. Therefore, the effects of short-chain fatty acids (SCFA), pH, ammonia, and urea on mRNA and protein levels of UT-B were assayed in primary rumen epithelial cell cu...