To investigate the relationship between major histocompatibility complex (MHC) class II compartments, secretory granules, and secretory lysosomes, we analyzed the localization and fate of MHC class II molecules in mast cells. In bone marrow-derived mast cells, the bulk of MHC class II molecules is contained in two distinct compartments, with features of both lysosomal compartments and secretory...

Sonicates of mouse bone marrow-derived mast cells (BMMC) differentiated in vitro and of mouse serosal mast cells differentiated in vivo contained small but approximately equal amounts of aminopeptidase activity, as determined by cleavage of leucine-beta-naphthylamide and resolution of the reaction products by reverse-phase high-performance liquid chromatography. Aminopeptidase activity was exoc...

OBJECTIVES Tuberculosis (TB) is the leading infectious disease in the developing world. Delayed-type hypersensitivity skin test diagnoses TB using tuberculin purified protein derivative (PPD), but this test is incapable of distinguishing Mycobacterium tuberculosis (MTB) infection from bacillus Calmette-Guérin (BCG) vaccination or an infection caused by nontuberculous mycobacteria (NTM). This st...

The finding that ingestion of antigens results in the selection induction of IgA antibodies in external secretions suggests that antigen sensitizes Peyer's patch lymphoid cells, which migrate to mucosal sites and generate local secretory IgA (S-IgA) antibody responses. Evidence for a common mucosal immune system in humans has been scanty because of the difficulty in demonstrating migratory beha...

BACKGROUND Although nitric oxide (NO) has been found to have a role in gut inflammation and to modulate immunoglobulin production, little is known about its part in food hypersensitivities. AIM This study aimed to evaluate the role of NO through the inhibition of constitutive and inducible NO synthase (cNOS and iNOS respectively) on the sensitisation process (antibody titres) and on intestina...

Background: Rat prostate gland which is used in various investigation has considerable morphological differences with other mammals. There are few reports about its structure. So, this study was carried on for determining of histomorphological structure of rat prostate. Materials and Methods: Five healthy adult male rats with average weight 20 ± 180 g and approximately 3 - 3.5 months age were s...

Investigations into the possibility that X (an antigen consistently present in aspirated odontogenic keratocysts, but not in most fluids from other cyst types), represented a keratinocyte component failed to identify the antigen as a keratin, involucrin, or one of the blood group substances. Antigen X was detected in human mixed and parotid saliva and in colostrum, as well as in a commercially ...

The effect of antiallergic agent, azelastine, was examined in immunogical secretory process of mast cells. 1. Azelastine significantly inhibited '"Ca uptake by mast cells stimulated by antigen, and the maximum inhibition was attained at a concentration of 50 11 11/me showing approximately 30.5 % inhibition. 2. Azelastine also inhibite the release of histamine from mast cells by antigen. The max...

This study was designed to compare the sensitivity and specificity of enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of ocular toxocariasis using Toxocara canis embryonated egg antigen (TEE) and toxocara excretory-secretory or exoantigen (TEX) produced in vitro. TEE and TEX ELISA were comparably sensitive, but TEX ELISA was better able to discriminate between serum samples from...

We produced a new monoclonal antibody (mAb) to the excretory-secretory (ES) antigens of Toxocara canis larvae. The mAb (IgG1) reacts specifically with the 120 kDa protein of many ES molecules and does not have any cross-reactivity with adult T. canis antigens. Sandwich ELISA to detect the ES antigens was performed using the mAb and rabbit polyclonal antiserum. The lower limit for the detection ...