fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. in this study, dot-elisa, one of the simplest and the most sensitive tests in this regard, was evaluated using excretory-secretory antigens of fasciola hepatica to diagnose human fasciolosis three groups consisting of patients infected with fasciolosis (n= 95), patients with o...

conclusions based on our study, in vivo prepared e/sa may be considered as a good candidate for animal immunization. results our results showed that the serum levels of anti-e/sa immunoglobulins significantly increased in all of the immunized groups. the differences of the serum levels of tgf-β between the groups were statistically significant at days 28 and 56 after immunization with e/sa. bac...

excretory/secretory antigen was prepared by culturing live adult worms of haemonchus contortus in rpmi 1640 medium at a concentration of 50 worms per ml in a culture-flask at 37 ˚c for 24 hr and the culture supernatant was used as antigen. the e/s antigen was purified by thiol-sepharose affinity chromatography. on western blot analysis, it was demonstrated that thiol-purified antigen showed a s...

background: ornithobilharziosis as one of the parasitic infections may give rise to serious economic problems in animal husbandry. the aim of the study was to prepare and compare the somatic and excretory-secretory (es) antigens of o. tur­kestanicum in gel diffusion test. methods: excretory-secretory (es) and somatic antigens of ornithobilharzia turkestanicum were prepared from collected worms ...

Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...

Background: Secretory proteins of IgE receptor activated mast cells and basophils play a pivotal role in the generation of immediate and long term immune responses in allergy and type I hypersensitivity. Objective: The present study aims to generate a 2-D map and profile of proteins secreted from a high secretory variant of the rat basophilic leukemia cell line, RBL-2H3.1, which in view of the ...

Excretory-secretory products of Toxocara canis larvae have been considered as a major functional antigen in immune responses against toxocariasis. We studied ultrastructural localization of T. canis second-stage larval antigen using a seropositive human serum under immunogold electron microscopy. High-density gold particles were observed in the secretory cells, excretory duct, intestinal epithe...

An immunoperoxidase technique was applied to formalin and Helly fixed paraffin wax sections from cases of ulcerative colitis complicated by dysplasia and carcinoma for carcinoembryonic antigen and components of the colonic secretory immunoglobulin system--namely, secretory component, IgA, and J chain. Sections from both resection specimens and mucosal biopsies were available. Intensity of immun...

background: secretory proteins of ige receptor activated mast cells and basophils play a pivotal role in the generation of immediate and long term immune responses in allergy and type i hypersensitivity. objective: the present study aims to generate a 2-d map and profile of proteins secreted from a high secretory variant of the rat basophilic leukemia cell line, rbl-2h3.1, which in view of the ...

Classical antigen presentation by major histocompatibility complex class I molecules involves cytosolic processing of endogenously synthesized antigens by proteasomes and translocation of processed peptides into the endoplasmic reticulum (ER) by transporters associated with antigen presentation (TAP). Alternative pathways for processing of endogenous antigens, generally involving the ER, have b...