Both polysomes and polysomal RNA, isolated from cotyledons of ripening pea (Pisum sativum) seeds and supplemented respectively with wheat germ S-100 and S-30 fractions, were used to program the cell-free synthesis of polypeptides. The relationship of these polypeptide products to seed storage proteins has been investigated. When fractionated on sucrose density gradients the translation products...

Plant proteins are plausible fishmeal substitutes but are deficient in some essential amino acids (EAA) like lysine and methionine. Combination of different plant proteins with complimentary EAA could be useful alternative. Bambaranut (Voandzeia subterranea) contains high amount of lysine while methionine is in sesame seed (Sesamum indicum). This experiment tested effects of combining sesame se...

UNLABELLED Soybeans are an important legume crop that contain 2 major storage proteins, β-conglycinin and glycinin, which account about 70- 80% of total seed proteins. These abundant proteins hinder the isolation and characterization of several low abundant proteins in soybean seeds. Several protein extraction methodologies were developed in our laboratory to decrease these abundant storage pro...

Allergic reactions to fruits and vegetables are frequently observed, however skin prick tests and serological analyses may not always confirm allergy. Recent advances have identified the seeds as potential allergen sources. Seeds are generally rich in storage proteins like 7S/11S globulins and 2S albumins, which are allergens known to be linked to severe reactions. For a number of plant food, s...

To gain insights into the protein dynamics during seed development, a proteomic study on the developing Brassica campestri L. seeds with embryos in different embryogenesis stages was carried out. The seed proteins at 10, 16, 20, 25 and 35 DAP (days after pollination), respectively, were separated using two-dimensional gel electrophoresis and identities of 209 spots with altered abundance were d...

In monocotyledonous plants, the process of seed development involves the deposition of reserves in the starchy endosperm and development of the embryo and aleurone layer. The final stages of seed development are accompanied by an increase in desiccation tolerance and drying out of the mature seed. We have used two-dimensional gel electrophoresis for a time-resolved study of the changes in prote...

Lysine, which is a limited amino acid in cereals, has a remarkably high content in buckwheat seed proteins. We have analyzed the pattern of 14C lysine incorporation into buckwheat seed polypeptides during the mid-maturation stage of seed development. Based on this approach, an 18 kDa polypeptide could be assigned as being lysine rich.

We profiled soybean and Arabidopsis methylomes from the globular stage through dormancy and germination to understand the role of methylation in seed formation. CHH methylation increases significantly during development throughout the entire seed, targets primarily transposable elements (TEs), is maintained during endoreduplication, and drops precipitously within the germinating seedling. By co...

Plant B3-domain transcription factors have an important role in regulating seed development, in particular seed maturation and germination. Among the B3 factors, the AFL (ABSCISIC ACID INSENSITIVE3 [ABI3], FUSCA3 [FUS3], and LEAFY COTYLEDON2 [LEC2]) proteins activate the seed maturation program in a complex network, while the VAL (VP1/ABI3-LIKE) 1/2/3 proteins suppress AFL action in order to in...