We report a case of a 38-year-old mold-allergic patient who developed episodes of generalized urticaria and systemic anaphylactic shock immediately after ingesting button mushrooms. A manganese-dependent superoxide dismutase (MnSOD) and a NADP-dependent mannitol dehydrogenase (MtDH) from Agaricus bisporus mushroom were identified as patient-specific IgE-binding proteins. Cross-reactivity betwee...

Allergies to certain fruits such as banana, avocado, chestnut and kiwi are described in 30-70% of latex-allergic patients. This association is attributed to the cross-reactivity between the major latex allergen hevein and hevein-like domains (HLDs) from fruit class I chitinases. We aimed to assess the extent of cross-reactivity between hevein and HLDs using sera from latex-allergic patients wit...

An IgM mAb (BB10) was produced by immunization of mice with human eosinophils purified according to their abnormal low density ("hypodense" cells), and previously shown to exhibit increased IgE-dependent antiparasite cytotoxicity. This BB10 antibody, selected for positive fluorescence staining of hypodense blood or lung eosinophils and low or negative staining of normodense eosinophils or neutr...

BACKGROUND Airborne Plantago pollen triggers respiratory allergies in Mediterranean countries. OBJECTIVES We aimed to study sensitization in patients with seasonal respiratory allergy and identify proteins of Plantago lanceolata pollen that could be responsible for hypersensitivity reactions in sensitized patients. We also determined the airborne pollen concentration of Plantago species from ...

The aim of the present study was to characterize allergen-specific immunoglobulin E (IgE) among children in Fez region. Eighty one children were recruited from the Hospital University Center of Fez. All of them had completed a questionnaire before taking sera. The sera were used to measure total IgE and specific data to proteins of egg's white (EWP), peanuts (PP) and gliadins (G). In last part,...

Allergic diseases are among the most common health issues worldwide. Specific immunotherapy has remained the only disease-modifying treatment, but it is not effective in all patients and may cause side effects. Over the last 25 years, allergen molecules from most prevalent allergen sources have been isolated and produced as recombinant proteins. Not only are these molecules useful in improved a...

of Vienna, Vienna, Austria; Floridsdorfer Allergiezentrum, Vienna, Austria. Background: Mung beans (Vigna radiata) contain 2 Bet v 1 related proteins: Vig r 1, a member of the PR-10 subfamily, and cytokinin-specific binding protein (CSBP), a protein with low sequence identity (31%) to Bet v 1. We aimed to compare Vig r 1 and CSBP to Bet v 1 regarding biochemical and immunological properties. Me...

of Vienna, Vienna, Austria; Floridsdorfer Allergiezentrum, Vienna, Austria. Background: Mung beans (Vigna radiata) contain 2 Bet v 1 related proteins: Vig r 1, a member of the PR-10 subfamily, and cytokinin-specific binding protein (CSBP), a protein with low sequence identity (31%) to Bet v 1. We aimed to compare Vig r 1 and CSBP to Bet v 1 regarding biochemical and immunological properties. Me...

It has been suggested that neutrophils may be involved in the late-phase reaction of immunoglobulin E (IgE)-dependent hypersensitivity states. However, the identity of neutrophil-associated molecules inducing the release of mediators remains unclear. In this report, we demonstrate that human neutrophils from normal donors or from patients with inflammatory disorders could bind myeloma IgE prote...

IgE Abs mediate allergic responses by binding to specific high affinity receptors (FcepsilonRI) on mast cells and basophils. Therefore, the IgE/FcepsilonRI interaction is a target for clinical intervention in allergic disease. An anti-IgE mAb, termed BSW17, is nonanaphylactogenic, although recognizing IgE bound to FcepsilonRI, and interferes with binding of IgE to FcepsilonRI. Thus, BSW17 repre...