Genetic study of pistachio, especially male genotypes due to the effects of pollen on nut quality and quantity and next generation characterizations, help to improve its management and breeding programs. We studied the genetic diversity among 20 male and 36 female pistachio genotypes using ISSR marker. In total 178 DNA frgments were proliferated using 12 primers that 169 fragments were polymorp...

Background: The dried sclerotium of medicinal fungus Polyporus umbellatus (Pers.) Fries has many pharmacological functions such as diuretic and anticancer activity, in which high-content polysaccharides may play an important role. However, RNA isolation is difficult in filamentous fungi and lacking in P. umbellatus. Results: Five methods for RNA extraction from five strains collected from four ...

Fruit shape is one of the most important quality factors in many domesticated plants. In this study, we used bulked segregate analysis (BSA) to identify two sequence-related amplified polymorphism (SRAP) markers linked to fruit shape in cucumber (Cucumis sativus L.). A segregating F2 population of 130 plants from a cross (B-2-2×Y-3) of B-2-2 with long fruit shape trait and Y-3 with round fruit ...

Determining and improving drought-tolerant cultivars is a major goal of plant breeding to face climate change. The productivity faba bean in Egypt affected by abiotic stresses, especially drought stress. This study evaluated eight Egyptian for tolerance under three soil water regimes consisting well-watered (100% field capacity), moderate stress (50% severe (25% capacity) pots greenhouse condit...

Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer combinations, and 10 ISSR primers, among whi...

Sequence-related amplified polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multiflorum Lam.). Eighteen SRAP primer combinations generated 334 amplification bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1)...

This study was conducted for molecular and morphological characterization of 28 local eggplant genotypes (so-called “Yamula” eggplant) collected from Kayseri province, 3 Kemer eggplant, 1 Manisa genotype, commonly cultivated in Turkey. Molecular analyses were carried out with the use 10 ISSR SRAP primers 30 characteristics. Morphological revealed nearest as ERU 3006 - 3007. The polymorphism rat...