objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These  embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0....

Abstract Tomato mottle mosaic virus (ToMMV) poses a threat to production and quality of tomato fruits. The Tm-2 2 gene confers resistance some tobamoviruses by recognizing viral movement proteins. However, -mediated against ToMMV is not well known. Here, we found that could infect wild-type but transgenic Nicotiana benthamiana plants also cultivar Moneymaker resistant Jili with homozygous . Chi...

background: adhesion and biofilm formation are two important steps in candida pathogenesis. the aim of the current study was to investigate the presence of bcr1 gene in candida albicans (c. albicans) isolates from women with vaginal candidiasis and its impact on biofilm formation. methods: we used 50 clinical isolates which confirmed c. albicans by pcr-rflp. then total rna was extracted from c....