In present study, a DLLME-HPLC-UV method was developed and validated for the extraction, pre–concentration, and subsequently quantification of vitamin D3 (Vit D3) in milk and yogurt samples. In order to be able to extract Vit D3 from studied samples efficiently, the DLLME procedure was optimized with respect to the parameters affecting the extraction efficacy, w...

Chen G, Yang Y, Fröhlich O, Klein JD, Sands JM. Suppression subtractive hybridization analysis of low-protein dietand vitamin D-induced gene expression from rat kidney inner medullary base. Physiol Genomics 41: 203–211, 2010. First published March 2, 2010; doi:10.1152/physiolgenomics.00129.2009.—Protein restriction and hypercalcemia result in a urinary concentrating defect in rats and humans. P...

BACKGROUND Scleroderma is a chronic connective tissue disease of unknown etiology. Vitamin D and parathyroid hormone (PTH) that play particular functions in calcium and phosphate homeostasis may be involved in the etiology of this disorder. Klotho, the co-receptor of the fibroblast growth factor 23 (FGF-23), can interfere with calcium and phosphate metabolism. The purpose of this study was to e...

The current studies were designed to characterize type IIb sodium-phosphate cotransporter (NaPi-IIb) expression and to assess the effect of 1,25-(OH)2 vitamin D3 (vit-D3) on NaPi-IIb gene expression during rat ontogeny. Sodium-dependent Pi absorption by intestinal brush-border membrane vesicles (BBMV) decreases with age, and NaPi-IIb gene expression also decreases proportionally with age. Vit-D...

OBJECTIVE To determine whether serum vitamin D levels are correlated with serum levels of alkaline phosphatase or not. STUDY DESIGN Cross-sectional, observational study. PLACE AND DURATION OF STUDY Multi-centre study, conducted at Liaquat National Hospital and Medical College, National Medical Centre and Medicare Hospital, Karachi, from January to October 2009. METHODOLOGY Patients attend...

The objective of this study was to determine the efficacy of supranutritional dietary selenium and vitamin E (Vit E) to ameliorate the effect of heat stress (HS) on oxidative status and acid-base balance in sheep. Thirty-two Merino × Poll Dorset ewes were acclimated to indoor individual pen feeding of a pelleted control diet (0.24 g Se and 10 IU of Vit E/kg DM) for 1 wk. Sheep were then moved t...

background: scleroderma is a chronic connective tissue disease of unknown etiology. vitamin d and parathyroid hormone (pth) that play particular functions in calcium and phosphate homeostasis may be involved in the etiology of this disorder. klotho, the co-receptor of the fibroblast growth factor 23 (fgf-23), can interfere with calcium and phosphate metabolism. the purpose of this study was to ...

BACKGROUND Arsenic trioxide (ATO) is a novel form of therapy that has been found to aid acute promyelocytic leukemia (APL) patients. Our laboratory has demonstrated that ATO-induced cytotoxicity in human leukemia (HL-60) cells is mediated by oxidative stress. Pro-oxidants have been known to play a role in free radical-mediated oxidative stress. Vitamin D3, (Vit D3) an active metabolite of vitam...