The suitability of an automated patch clamp for the characterization and pharmacological screening of calcium release-activated calcium (CRAC) channels endogenously expressed in RBL-2H3 cells was explored with the QPatch system. CRAC currents (I( CRAC)) are small, and thus precise recordings require high signal-to-noise ratios obtained by high seal resistances. Automated whole-cell establishmen...

background: ketamine is a commonly used intravenous anesthetic which produces dissociation anesthesia, analgesia, and amnesia. the mechanism of ketamine-induced synaptic inhibition in high-level cortical areas is still unknown. we aimed to elucidate the effects of different concentrations of ketamine on the glutamatergic synaptic transmission of the neurons in the primary somatosensory cortex b...

Buthotus schach is one of the dangers scorpion in Iran that belong to the Buthidae family. Toxins are existing in venom scorpion, modulate the ion channels by blocking or opening the pore of the channel or by altering the voltage gating and useful as pharmacological tools. In the present study, we investigated the effect of venom and its obtained fractions by gel filtrations on electrophysiolog...

Results Using insulin and glucagon secretion protocols with fresh islets isolated from C57BL6 mice, we observed that CORT reduced the glucose-stimulated insulin secretion (GSIS) in a similar magnitude from that of SST (p<0.01), an effect mediated by SST-R5 receptor. Glucagon secretion in response to 0.5 mM glucose was completely abrogated in the presence of CORT (p<0.001), as well as for SST. B...

Hypoxia is a common factor of numerous ocular diseases that lead to dysfunctions and loss retinal ganglion cells (RGCs) with subsequent vision loss. High-voltage-activated calcium channels are the main source entry into neurons. Their activity plays central role in different signaling processes health diseases, such as enzyme activation, gene transcription, synaptic transmission, or onset cell ...

Background: The consideration of neurons as coupled mechanical-electrophysiological systems is supported by a growing body experimental evidence, including observations that cell membranes mechanically deform during the propagation an action potential. However, short-term (seconds to minutes) influence membrane voltage on mechanical properties neuron at single-cell level remains unknown. Materi...

Recent advances in neuronal culturing techniques have supplied a new set of tools for studying neural tissue, providing effective means to study molecular aspects of regulatory elements in the supraoptic nucleus of the hypothalamus (SON). To combine molecular biology techniques with electrophysiological recording, we modified an organotypic culture protocol to permit transfection and whole cell...

Potassium channels are trans-membrane proteins, which selectively transport K+ ions across cell membranes and play a key role in regulating the physiology of excitability cells and signal transduction pathways. Bothutous Schach (BS) scorpion venom consists of several polypeptides that could modulate ion channels. In this study, the effects of BS crude venom on passive and active electrophysiolo...