Mycobacterium tuberculosis, the main cause of tuberculosis (TB), has still remained a global health crisis especially in developing countries. Tuberculosis treatment is a laborious and lengthy process with high risk of non compliance, cytotoxicity adverse events and drug resistance in patient. Recently, there has been an alarming rise of drug resistant in TB. In this regard, it is an unmet need...

Background: Antioxidants are developed to assist the immune system and overcome oxidative stress, the aggression of cellular constituents due to imbalance between reactive oxygen species and the inner antioxidant system. The main objective of this study was to search for new and potent antioxidants to protect humans against diseases associated with oxidative stress. Methods: In this study, thr...

Accumulating evidence indicates that toll-like receptor 4 (TLR4) plays a critical role in promoting adaptive immune responses and are definitively involved in the expansion and maintenance of the neuropathic pain. However, the application of docking in virtual-screening in silico methods to drug discovery has some challenge but it allows us to make the directed and meaningful design of drugs fo...

Objective(s): We previously conducted an in silico research on the interactions between the ribosome display-selected single chain variable fragment (scFv) and factor H binding protein (fHbp) of Neisseria meningitidis. We found that heavy chain variable (VH) fragment of this scFv had considerable affinity to fHbp. These results led us to evaluate the ability of this sm...

RNAi mechanism plays a major role in silencing the expression of target genes by siRNAs. In the current study, in silico properties of 30 genes in omega-2 gliadin and 266 nt and 326 nt mutations were investigated before and after cloning in an expression vector. Specific primers were designed for 30 genes with spacer regions of 75 nt and 178 nt (for gene invert repeats). The frequency of siRNA ...

globally, brucella melitensis and b. abortus are the most common cause of human brucellosis. the outer membrane protein 31 (omp31) and l7/l12 are immunodominant and protective antigens conserved in human brucella pathogens which are considered as potential vaccine candidates. we aimed to design the fusion protein from brucella l7/l12 and truncated omp31proteins, in silico , clone the fusion in ...

Objective(s): Alzheimer’s disease (AD) as progressive cognitive decline and the most common form of dementia is due to degeneration of the cholinergic neurons in the brain. Therefore, administration of the acetylcholinesterase (AChE) inhibitors such as donepezil is the first choice for treatment of the AD. In the present study, we focused on the synthesis and anti-cholinesterase evaluation of n...

کانسار فسفات دلیر در 57 کلیومتری جنوب غربی شهر چالوس استان مازندران و زون البرز مرکزی دارد. میزبان این ذخیره سازند سلطانیه است که یکی از مهم‌ترین سازندهای رسوبی ایران است. نتایج آنالیز پراش اشعه ایکس (XRD) مطالعات میکروسکوپ نوری الکترونی بر روی نمونه‌های نشان داد آپاتیت، کلسیت، کوارتز دولومیت کانی‌های اصلی فرعی شامل مونتموریلونیت، پیریت باریت مقادیر به همراه جزیی سیدریت، روتیل، ایلیت گوتیت هستن...

Protein tertiary structures exhibit an observable degeneracy in nature. This paper examines the connection between a protein motif’s abundance in nature and its designability as measured by in silico methods. After generating a set of protein structures, we evaluated each structure’s abundance in nature, ratio of possible contacts (contact degree) and in silico designability. Our results showed...