Cotyledonary node explants excised from 21 day old seedlings of T. arjuna produced multiple shoots when cultured on full strength MS or modified MS (1/2 strength major salts and Fe-EDTA) medium supplemented with different concentrations (0.1-1.0 mg/l) of BAP. Maximum 8.9 shoots/explant could be recorded after 30 days of inoculation on modified MS medium supplemented with BAP (0.5 mg/l). A proli...

Methanolic extracts from in vitro grown Scutellaria lateriflora shoots cultured on five Murashige and Skoog (MS) medium variants supplemented with different combinations of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA) under different light conditions (monochromatic light, white light and no light) were analysed by HPLC for three groups of metabolites: flavonoids (26 compounds), ...

The first experiment was done to select the most suitable explant establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP 0.2 NAA. Cotyledon responsive which exhibited quick callus initiation. second conducted determine effect of media BAP, NAA a...

In vitro flowering and effective micropropagation protocol were studied in Swertia chirayita, an important medicinal plant using axillary bud explants. The Murashige and Skoog's medium (MS) supplemented with benzyl amino purine (BAP) 1.0 mg L(-1) and adenine sulfate 70.0 mg L(-1) was found optimum for production of multiple shoots. In the present study, incubation of flowering cultures on BAP s...

Article info: Received: 9 April 2014 Accepted: 6 August 2014 ABSTRACT An effective in vitro culture system for mature nodal segments of European plum (Prunus domestica) was established. Several important aspects of regeneration from nodal explants were studied for adaptation of European plum in Bangladesh. Leaf explants were used for callus induction and 1⁄2 MS media supplemented with 1.0 mg/l ...

Ridge gourd (Luffa acutangula L. Roxb) is a popular and nutritious vegetable in Bangladesh. The present investigation was undertaken to establish an efficient in vitro regeneration system of ridge gourd from immature embryo and cotyledon explants. Immature embryos and cotyledons were collected from immature seeds and culture on MS medium supplemented with various concentration and combination o...

BACKGROUND Aloe vera L. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. Aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income. OBJECTIVES In this study, the effect of different media on shoot proliferation of the shoot tip of Aloe vera L. was investigated. MATERIALS AND ...