ribosomal proteins and @ H-labeled growing chains were mixed and dually counted after fractionation by different techniques. Ehrlich adenocarcinoma ascites cells were taken from mice (Swiss strain) inoculated 6 days earlier. The cells were separated from the ascitic plasma by centrifugation at 500 X g for S mm and washed once with 3 volumes of a medium containing NaC1 (0.12 M), KC1 (13 mM), MgS...

Abstract Background Rhodosporidium strain , a well-known oleaginous yeast, has been widely used as platform for lipid and carotenoid production. However, the production of squalene application in lipid-based biofuels is not reported this strain. Here, new sp. was isolated identified, its potential investigated under various cultivation conditions. Results In present study, DR37 from mangrove ec...

Callus was obtained from leaf explants of Cephaelis ipecacuanha on Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/l Kin, 4.0 mg/l 2,4-D and 3% (w/v) sucrose [Murashige, T., Skoog, F., 1962. Plant Physiol. 15, 437±497]. Plantlet regeneration through somatic embryogenesis was achieved on MS medium containing 2.5 mg/l Kin, 1.0 mg/l 2,4-D and 3% (w/v) sucrose. Maturation and germina...

Abstract Abiotic stresses are the most major limitation to crop production, causing yield losses up 50%, and consider challenges for production of crops. Thus, an experiment was conducted determine how sugar beet would respond soil application K-humate rates foliar biostimulants under salt stress. Split-plot design in three replicates used both seasons, where fertigation (control, 12, 24 kg ha ...

Two trials were conducted to identify the optimal levels of essential oil active components (EOAC) and their combination with fumarate on in vitro rumen fermentation. In trial 1, eugenol, carvacrol, citral and cinnamaldehyde were mixed at ratios of 1:2:3:4, 2:1:4:3, 3:4:1:2, 4:3:2:1 and 1:1:1:1 to make up five combinations (EOAC1, EOAC2, EOAC3, EOAC4 and EOAC5 respectively). The mixtures were s...

INTRODUCTION To control the tissue browning phenomenon, callus growth, total phenolics and paclitaxel production, in the current investigation, we evaluated the effects of citric acid and ascorbic acid (as antioxidants) and glucose, fructose and sucrose in callus cultures of Taxus brevifolia. METHODS To obtain healthy callus/cell lines of Taxus brevifolia, the effects of two antioxidants asco...

Objective The preventive effect of Zataria multiflora in animal models of asthma has been reported. In the present study, its effect on wheezing, forced expiratory volume in 1 second, and plasma nitrite (NO2-) in asthmatic patients was studied. Materials and Methods In this study, forty asthmatic patients including 15 males and 25 females (aged 45.8±6.21 years) were randomly allocated in four...

Two strains of callus have been isolated from bean hypocotyl and grown on a defined maintenance medium supplemented with 2 mg/l. 2:4-dichlorophenoxyacetic acid (2:4D) and 2% sucrose. Root initiation was observed in one strain and formation of nodules containing xylem and phloem in both strains after transfer to an induction medium supplemented with 1 mg/l. naphthyleneacetic acid, 0-2 mg/l. kine...

The present work aimed to investigate the effects of acute sucrose treatment on the perception of painful stimuli. Specifically, we sought to determine the involvement of the endogenous opioid peptide-mediated system as well as the role of the μ(1)-opioid receptor in antinociception organisation induced by acute sucrose intake. Nociception was assessed with the tail-flick test in rats (75, 150 ...