Intracellular Ca2+ (Ca2+i) transients during fertilization are critical to the activation of eggs in all species studied. Activation of both the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) and ryanodine receptor (RYR) are responsible for the calcium oscillations during fertilization in sea urchin eggs. Using in vitro matured bovine oocytes loaded with Fura-2 AM ester as Ca2+i indicator, ...

Due to narrow genetic base of groundnut (Arachis hypogaea L.), induction of genetic variability was undertaken by treating seeds of TFDRG 5 with 200 and 300 Gy of gamma rays and with 1, 2 and 3 mM of sodium azide (NaN 3 ) singly and in combination. The combination of 200 Gy and 3 mM of NaN 3 induced maximum number of mutants with a frequency of 2.48% in M 2 generation. In all, 63 true breeding ...

Purpose: To explore how the selection of threshold b-value impacts Intravoxel Incoherent Motion (IVIM) diffusion parameters of PF (f), D slow (D), and D fast (D*) values and their performance for liver fibrosis detection. Materials and Methods: Fifteen healthy volunteers and 33 hepatitis-b patients were included. With a 1.5 T MR scanner and respiration gating, IVIM data was acquired with 10 b-v...

The objective of this experiment was to determine the effect of prerigor injection of several glycolytic inhibitors on pH, color, tenderness, and related traits of low-value beef cuts. The semimembranosus, triceps brachii, and supraspinatus muscles from each of 10 steer carcasses were removed 1 h postmortem. Control samples remained in the carcass at 2 degrees C for 24 h. Prerigor muscles were ...