Three new rimantadine Schiff bases (RSBs) were prepared, and then the interaction of RSBs with bovine serum albumin (BSA) was investigated using fluorescence, synchronous fluorescence, UV-vis absorption spectroscopy under physiological conditions. The results showed that the three RSBs effectively quenched the intrinsic fluorescence of BSA via static quenching. Binding constant (Ka), number of ...

By coupling 5-Methyl-cytidine to bovine serum albumin, a conjugate containing 18 mol of 5-Methyl-cytidine per mol of BSA was prepared. Antibodies to this conjugate were produced by immunization of rabbits. Specificity of the antibody was assessed by gel diffusion in agar containing excessive amounts of the carrier BSA. A slight cross-reactivity with cytidine was eliminated by adsorption on the ...

The color is a sensory attribute that influence decisively the judgment of consumers in certain type of food and therefore, the food industries have added dyes to their products. Natural dyes have been demanded by consumers but there are some limitations in its use such as low water solubility and low stability. Therefore, we have sought ways of overcoming these limitations and milk proteins pl...

The incorporation of a model protein, bovine serum albumin (BSA), into poly(vinyl alcohol) (PVA) hydrogel films during the freezing and thawing process and its subsequent release behavior were investigated. The effect of the number of freezing and thawing cycles as well as the stability of BSA were examined. BSA release profiles were not significantly different from gels prepared after 3 or 5 c...

Toxic epidermal necrolysis (TEN) is a severe adverse cutaneous drug reaction characterized by mucosal erosion and epidermal detachment [1]. It is usually distinguished from Stevens-Johnson Syndrome (SJS) by the extent of affected body surface area (BSA): SJS when less than 10% of BSA is affected, TEN when BSA affected is more than 30% and when 10 to 30% of BSA is affected as SJS-TEN overlap [1,...

The aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºC for 0, 12, 24, 48 and 72 hrs after collection anddilution. A total of 57 ejaculates from 12 Bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following an...

The interactions of 3-carboxyphenoxathiin with Bovine Serum Albumin (BSA) and Human Serum Albumin (HSA) have been studied by fluorescence and circular dichroism spectroscopy. The binding of 3-carboxyphenoxathiin quenches the BSA and HSA fluorescence, revealing a 1:1 interaction with a binding constant of about 10(5) M(-1). In addition, according to the synchronous fluorescence spectra of BSA an...

The interaction between bovine serum albumin (BSA) and methyl cinnamate (MC), an strawberry flavor compound, was investigated. We found that BSA and MC form complex with stoichiometry (n) equal 1, driven by system’s entropy increase and enthalpy reduce at pH 7.4, 5.0 and 3.5, with binding constants (Kb) in magnitude of 10 5 L.mol -1 . There was difference in variation of standard enthalpy of co...