In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. We focused on induction of the acrosome reaction as a key process in fertilization. Six species of sea stars were used in this study: Asterias amurensis, Asterias rubens, Asterias forbesi, Aphelasterias japonica, Distolasterias nipon, and Asterina pectinifera. Acrosome reaction ...

The extracellular coat, or zona pellucida, of mammalian eggs contains species-specific receptors to which sperm bind as a prelude to fertilization. In mice, ZP3, one of only three zona pellucida glycoproteins, serves as sperm receptor. Acrosome-intact, but not acrosome-reacted, mouse sperm recognize and interact with specific O-linked oligosaccharides of ZP3 resulting in sperm-egg binding. Bind...

Objective The aim of this study was to investigate the effects of curcumin on epididymal sperm parameters in adult male Navel Medical Research Institute (NMRI) mice exposed to sodium arsenite. MaterialsAndMethods In this experimental study, we divided the animals into four groups: control, sodium arsenite (5 mg/kg), curcumin (100 mg/kg) and curcumin+sodium arsenite. Exposures were performed by ...

Mammalian spermatozoa are extremely susceptible to high doses of reactive oxygen species (ROS). The aim of the present study was to investigate the potential role of glutamine in protecting rabbit spermatozoa against ROS stress during cryopreservation and post-thaw incubation. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with glutamine. The addition of 20...

Capacitation and the acrosome reaction of mammalian spermatozoa are essential for fertilization. In vitro results are presented that demonstrate that catecholamines stimulate activation (a whiplash flagellar movement characteristic of capacitated hamster spermatozoa) and the acrosome reaction. Protein-free ultrafiltrates of bovine adrenal cortex and medulla preparations stimulated motility, act...

this experiment was conducted to evaluate the addition of seminal plasma to coated spermatozoa after 72 h. semen was collected from four taleshi rams by artificial vagina. to prepare coated spermatozoa, semen was collected in tube containing tris-fructose-15% egg yolk. after initial evaluation, the samples were mixed and centrifuged. after removal of supernatant and dilution, samples were divid...

.................................................................................................vii CHAPTER 1: INTRODUCTION..........................................................................1 CHAPTER 2: LITERATURE REVIEW.................................................................3 2.1. Stallion Spermatozoa........................................................................3 2.1...

Bull spermatozoa found in the cow genital tract 18 hours postcoitum are observed in situ by both light and electronic microscopy after the uterotubal junction and its surrounding area are fixed. No phagocytosis is evident then, and many spermatozoa are seen in the folds of the uterotubal junction mucosa. None of the spermatozoa evidence ultrastructural integrity. Some show only periacrosomal pl...

Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage chromatin. Therefore, quality was compared in samples following single-layer centrifugation (SLC) or and controls (extension only) two extenders, INRA82 INRA96. Ejaculates from six stallions were split among treatments: SLC, washing, controls, Sperm motility acrosome status evaluated at 0, 24, 4...

Effect of two antioxidants (Taurine and Manganese) in extender on different spermparameters (sperm motility, live sperm percentage, membrane acrosome integrity) Sahiwal bullswas evaluated. Semen was collected twice a week for 8 weeks from six adult, regular donor andclinically healthy bulls. A total 96 ejaculates were pooled after initialevaluation. The semen samples diluted at with tris-citric...