in this work, experimental kinetics data of methane hydrate decomposition at temperatures ranging from 272.15 to 276.15 k and at pressures ranging from 10 to 30 bars were modeled by using chemical affinity. this model proposed a macroscopic model which is independent of any intermediate mechanism like heat or mass transfer. the results show there is good agreement with experimental data. also t...

in this study escherichia coli de3 containing expression vector (pet21a) with cloned persian sturgeon growth hormone (psgh) gene was grown in 10 ml lb broth on a 150 rpm shaker, at the temperature of 37 °c. at the late log phase (determined by od standard curve) 100 μl isopropyl β-d-1-thiogalactopyranoside (iptg) was added for induction of gh synthesis. samples were taken every 2 hours and afte...

the purification of biomolecules is a necessary step in many biochemical researches. in this regard, developments of convenient, specific and low cost methods of purification are of particular interest. given the human hemoglobin (hb) affinity toward some charged carbohydrates, interaction of this molecule with human chorionic gonadotropin (hcg) which is a glycoprotein hormone containing sialic...

Hydrophobicity properties of graphite and green synthesized graphene (gsG) from exfoliated graphite/GO towards polymer membrane characteristic and properties at different weight percentage concentrations (1, 2, 3, 4 and 5 wt. %) were investigated. PSf/graphite and PSf/gsG membranes were characterized in term of hydrophobicity, surface bonding, surface roughness and porosity. FTIR peaks revealed...

introduction: the major histocompatibility complex (mhc) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. the physiologic function of mhc molecules is the presentation of peptides to t cells. in this study, we evaluated the purification of a class ii mhc molecule (hla-dr) from a human burkitt′s lymphoma cell line; daudi. materi...

introduction: polyclonal antibodies are required to be affinity purified. improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. two common techniques for purifying proteins is salt precipitation and chromatography purification. our work fo...