نتایج جستجو برای: bacmid shuttle vector

تعداد نتایج: 203458  

2017
Takashi Dojima Takuya Nishina Tatsuya Kato Tsuyoshi Uno Koichi Kato Hiroshi Ueda Enoch Y. Park

Human 29IJ6 IgG was expressed in silkworm using a Bombyx mori 25 nucleopolyhedrovirus (BmNPV) bacmid system. The mean amounts of 296IJ6 IgG 26 produced in larval hemolymph and whole pupae were 30.1 μg/larva and 78.0 μg/pupa,

Journal: :novelty in biomedicine 0
mehdi shokri immunology department and virology department, pasteur institute of iran, tehran, iran soheila ajdary immunology department, pasteur institute of iran, tehran, iran mina ebrahimi-rad biochemistry department, pasteur institute of iran, tehran, iran arash memarnejadian hepatitis and aids department, pasteur institute of iran, tehran, iran mohamad-hossein alimohamadian immunology department, pasteur institute of iran, tehran, iran fatemeh motevali hepatitis and aids department, pasteur institute of iran, tehran, iran

background: leishmania major lmsti1 is a conserved protein among different species of leishmania, and expressed in both amastigote and promastigote forms of l. major life cycle. it has previously been expressed in bacterial systems. m aterials and methods: to express lmsti1 in the methylotrophic yeast         pichia pastoris ( p. pastoris ), the shuttle vector ppicza containing gene lmsti1 was ...

Journal: :Virus research 2008
Oihane Simón Trevor Williams Primitivo Caballero Robert D Possee

Homologs to Autographa californica multiple nucleopolyhedrovirus (AcMNPV) open reading frame (ORF) 136 or Acp26 are present within almost all nucleopolyhedroviruses (NPVs). Two copies of the gene are found in some members of group II NPVs, suggesting that it may play an important role in transmission or replication. Phylogenetic analysis revealed that the predicted protein has some similarity w...

Journal: :Journal of virology 2010
Wenbi Wu A Lorena Passarelli

The Autographa californica multiple nucleopolyhedrovirus orf92 (p33), ac92, is one of 31 genes carried in all sequenced baculovirus genomes, thus suggesting an essential function. Ac92 has homology to the family of flavin adenine dinucleotide-linked sulfhydryl oxidases and is related to the ERV/ALR family of sulfhydryl oxidases. The role of ac92 during virus replication is unknown. Ac92 was ass...

Faranak Ghazi Sherbaf Majid Motovali-Bashi, Zohreh Hojati,

The claR of Streptomyces clavuligerus in the clavulanic acid gene cluster encodes a transcriptional regulator that controls clavulanic acid biosynthesis. The main goal of this study was isolation and molecular detection of the claR gene and its cloning in the Streptomyces specific vector (pMA:: hyg). By cinsideration of the claR gene’s start codon, the specific primers were designed. After geno...

2017
Byoung-Jun Kim Jeong-Ryeol Gong Ga-Na Kim Bo-Ram Kim So-Young Lee Yoon-Hoh Kook Bum-Joon Kim

Recently, we have developed a novel Mycobacterium-Escherichia coli shuttle vector system using pMyong2, which can provide an enhanced expression of heterologous genes in recombinant Mycobacterium smegmatis (rSmeg). To investigate the usefulness of rSmeg using pMyong2 in vaccine application, we vaccinated M. smegmatis with pMyong2 system expressing Human Immunodeficiency Virus Type I (HIV-1) Gag...

Journal: :Applied and environmental microbiology 2004
Maryvonne Arnaud Arnaud Chastanet Michel Débarbouillé

A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The ...

Journal: :Journal of virology 1995
C Giraud E Winocour K I Berns

A model system using an episomal Epstein-Barr virus shuttle vector was recently developed to study the adeno-associated virus (AAV) site-specific integration event in chromosome 19q13.3-qter (C. Giraud, E. Winocour, and K.I. Berns, Proc. Natl. Acad. Sci. USA 91:10039-10043, 1994). In this study, we analyze the recombinant junctions generated after integration of the AAV genome into an Epstein-B...

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