نتایج جستجو برای: bacmid shuttle vector

تعداد نتایج: 203458  

Journal: :iranian journal of virology 0
mr shafaati department of microbiology, islamic azad university, damaghan branch, damaghan, iran m moghbeli department of microbiology, islamic azad university, damaghan branch, damaghan, iran r dorostkar applied virology research center, department of virology, baqiyatalah university of medical sciences, tehran, iran

background and aims: newcastle disease virus (ndv) is one of the major pathogen in poultry. vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. f gene open reading frame (orf) from ndv is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. the f glycoprotein on the surf...

2011
S Hossain Zadeh F Fotouhi MT Kheiri MR Razavi B Heydarchi B Farahmand S Najafi

Influenza virus A (H1N1) is an important subtype of influenza virus that makes numerous consequences throughout the world. In the first step of viral attachment, main antigenic site of the HA1 domain from the globular head of hemagglutinin (HA) binds to human cell receptors, starting the disease process. In order to produce recombinant subunit protein vaccines, we focus on the nucleotide sequen...

Journal: :modares journal of medical sciences: pathobiology 2012
samaneh hossainzadeh fatemeh fotouhi behrokh farahmand maryam saleh atena yousefi

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Majid Moghbeli , Mohammad reza Shafaati , Roholah Dorostkar,

Background and Aims: Newcastle disease virus (NDV) is one of the major pathogen in poultry. Vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. F gene open reading frame (ORF) from NDV is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. The F glycoprotein on the surf...

Journal: :BioTechniques 2000
A V Folgueras-Flatschart R B Flatschart M Resende M C Sogayar

The most widely studied baculovirus, the Autographa californica M nucleopolyhedrovirus (AcMNPV), is a large, enveloped DNA virus that infects lepidopteran larvae (8). This virus is commonly used as a eukaryotic vector to express heterologous genes in cultured insect cells and insect larvae (1,3,15). Baculovirus expression systems offer several advantages over other expression systems, among whi...

Journal: Vaccine Research 2015
E Akhavan , M Shafaati , MR Shafaati , SH Yazdani ,

Introduction: In recent years, influenza viruses have caused moderate to severe infections all around the world while so far there is no influenza vaccine that can protect people with only one dose of injection. In this regard, producing a universal vaccine based on virus-like-particles (VLP) could be an ideal approach.  Methods: In this study, the full-length ORF of influenza hemagglutini...

Journal: :Journal of biochemistry and molecular biology 2005
Qin Qin Ying-le Liu Ying Zhu Shun-yi Li Yi-peng Qi

In this study we developed the transposon-mediated shuttle vector 'Hanpvid', which composed of HaNPV (Heliothis armigera nuclear polyhedrosis virus) genomic DNA and a transposon cassette from Bacmid of Bac-to-Bac system. Hanpvid replicates in E. coli in the same way as Bacmid and retains infective function in cotton bollworm cells (Hz-AM1). Using Hanpvid we constructed a recombinant virus, whic...

Journal: :Bioscience reports 2009
Wu-Jie Su Wei-De Shen Bing Li Yan Wu Guang Gao Wen-Bing Wang

In the present study, we studied the feasibility of deleting essential genes in insect cells by using bacmid and purifying recombinant bacmid in Escherichia coli DH10B cells. To disrupt the orf4 (open reading frame 4) gene of BmNPV [Bm (Bombyx mori) nuclear polyhedrosis virus], a transfer vector was constructed and co-transfected with BmNPV bacmid into Bm cells. Three passages of viruses were c...

Journal: :vaccine research 0
mr shafaati department of cellular & molecular biology iau, hamadan branch, hamadan, iran. e akhavan department of microbiology iau, damaghan branch, damaghan, semnan, iran. sh yazdani department of virology, school of public health, tehran university of medical sciences, tehran, iran. m shafaati department of microbiology iau, jahrom branch, jahrom, shiraz, iran.

introduction: in recent years, influenza viruses have caused moderate to severe infections all around the world while so far there is no influenza vaccine that can protect people with only one dose of injection. in this regard, producing a universal vaccine based on virus-like-particles (vlp) could be an ideal approach.  methods: in this study, the full-length orf of influenza hemagglutinin (ha...

Journal: :Polish journal of microbiology 2008
Wu-Jie Su Yan Wu Hui-Ling Wu Shan-Ying Zhu Wen-Bing Wang

Open reading frame 76 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm76, is a gene whose function is completely unknown. With EGFP fused to the 3' terminal of Bm76 as the reporter gene and BmNPV bacmid as the expression vector, a recombinant bacmid was successfully constructed expressing Bm76-EGFP fusion protein under the control of polyhedrin promoter in Bombyx mori cells (Bm cel...

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