In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10-15 year old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) suppleme...

Cell-suspension cultures of Capsicum annuum L., Solanum aculeatissimum Jacq. and, Datura fastuosa L. (Solanaceae) were established and investigated for the ability to biotransform exogenously-supplied hydroquinone to arbutin. All cultures exhibited good 24-day growth in Murashige and Skoog medium, supplemented with 2,4-dichlorophenoxyacetic acid and benzylaminopurine as plant growth regulators,...

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic em...

A number of compounds have been reported to be specific inhibitors of protein kinases mediated by structural-based selectivity, but the development of specific inhibitors has not yet been addressed in plant science. Here we tested C2, C6, and N9-trisubstituted purines to determine the basic relationship between their chemical structure and inhibitory activity versus a plant mitogen-activated pr...

A simple micropropagation method from shoot tip explants was reported here for Clitoria ternatea L., an economically important medicinal plant. High frequency of micro shoots were obtained from this explants on MS medium supplemented with various concentrations of Indole Acetic acid (IAA) (0.5 2.0 mg/l) and KN (0.5 -2.0 mg/l). Maximum number of shoots was obtained from shoot tip explants in the...

Withania somnifera (L.) Dunal is high demanding valuable medicinal plants which have pharmaceuticals properties, known for its with anolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog’s, 1969) medium horizontally containing different concentration(1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axil...

A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3% (w/v) sucrose. The rate of multip...

Aim. The effect of chronic ultraviolet B (UV-B) radiation and cytokinin benzylaminopurine (6-BAP) on the growth, content photosynthetic pigments hydrogen peroxide (HP) in leaves pea plants (Pisum sativum L.) was studied. Methods. Pea cultivar Gotivsky were irradiated by UV-B at a dose 3.5 kJ/m2 per day during 5 days. Groups non-irradiated treated with 6-BAP after end radiation. Length shoots ro...

A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our findings showed that stem meristems were easier to obtain, presented lower levels of contaminati...

Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 μM 2,4-dichlorophenoxyacetic acid, 1 μM 6-benzylaminopurine and 1 μM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive redu...