Of nine plant growth regulators (indoleacetic acid, 1-naphthalene acetic acid, gibberellic acid, giberellin 4/7, 6-benzylaminopurine, 6-furfurylaminopurine, abscisic acid, and 1-aminocyclopropane carboxylic acid) tested, only 6-benzylaminopurine and abscisic acid affected (14)C-photosynthate unloading from excised seed coats of Phaseolus vulgaris L. Unloading, in the presence of KCl, was stimul...

A simplified method for the determination of benzylaminopurine in agricultural products by LC/MS was investigated. Benzylaminopurine in agricultural products was extracted with acetone and the extract was concentrated to below 30 mL. Buffer solution of pH 9.0 and ethyl acetate were added to the residue, and the solution was shaken. The ethyl acetate layer was evaporated to dryness, and the resi...

Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasm...

The effect of benzylaminopurine was studied on cyanide-resistant mitochondria isolated from aged slices of potato tuber (Solanum tuberosum L. var. Bintje). Benzylaminopurine specifically acted on the cyanide-resistant alternative pathway. In the case of succinate oxidation, it mimicked the action of salicylhydroxamic acid and restored a good oxidative phosphorylation. Kinetic analyses showed th...

With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the...

germinated within 24 hours after prior exposure either to blue light or when maintained under complete darkness. However, far-red light augmented the germination of seeds pretreated with 6-benzylaminopurine so that nearly all such seeds germinated within 24 hours after treatment (table II). Far-red light also augmented the rate of germination of seed pretreated with a synergistic mixture of 6-b...

In breeding programs, a large number of F₂ individuals are required to perform the selection process properly, but often few such plants are available. In order to obtain more F₂ seeds, it is necessary to multiply the F₁ plants. We developed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of seeds using 6-benzylaminopurine. To optimize shoot regeneration...

Cryopreservation of medicinal plants is an important tool for restoration of their potential to synthesize valuable secondary metabolites. Establishment of an efficient protocol requires detailed understanding of species/genotype-specific responses to changing external variables. The aim of this work was to study the genotype-dependent response of Hypericum perforatum L. shoot tips to different...