Considering the ease of isolation and high expansion potential of pulp stem/progenitor cells isolated from wisdom and primary teeth they have been implicated as the most reliable autologous cell source in dental tissue engineering. Meanwhile, different isolation methods have remarkable impacts on the expansion potential of adult stem cells. In enzymatic digestion method extracted teeth as denta...

In the present investigation, four types of explants from mature zygotic embryos coconut, viz., whole upper cotyledonary region without haustorium, half plumule with a portion radicle and exclusively plumular tissue, were cultured in 12 different media combinations to find suitable explant which could be regenerated after cryopreservation. Explants pre-cultured medium 0.4 0.5 M sucrose for thre...

Many factors may influence the efficiency of somatic embryogenesis. This capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. The effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mM) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

Thymus daenensisis a perennial plant belonging to Lamiaceae family that is a species endemic to Iran. Due to the indiscriminate use of this plant which possesses medicinal values, T. daenensis tissue culture is suggested to increase the quality and quantity of its effective compounds. Callus cultures using leaf and stem explants were subjected to different hormonal treatments. Ten-millimeter ex...

The aim of the current in vitro study was to investigate if tissue surface modification with collagenase and addition of the TGF-β3 can increase the number of cells present in meniscus tears repaired with the use of newly developed tissue adhesives based on isocyanate-terminated block copolymers. Cylindrical explants were harvested from the inner part of bovine menisci. To simulate a full-thick...

Our objectives were to test differences in protein synthesis and secretion by cultured oviducts and endomemum from Brahman and Holstein cows and the response of those tissues to in vitro heat shock. Explants of oviductal tissue obtained at estrus from Holstein (n = 5 ) and Brahman (n = 6 ) cows were cultured at a homeothermic (39°C) or heat shock (43°C) temperature. At 6 h, cultures were pulse-...

The most immediate and evident effect of mucosal exposure to semen in vivo is a local release of proinflammatory mediators accompanied by an influx of leukocytes into the female genital mucosa (FGM). The implication of such response in HIV-1 transmission has never been addressed due to limitations of currently available experimental models. Using human tissue explants from the uterine cervix, w...

Here we describe a method for growing fibroblasts from human skin explants that increases the number of cells obtained by up to two orders of magnitude, thus increasing the amount of material available for research and diagnostic purposes and potentially for cell-based therapies. Explants can be transferred sequentially up to 80 times, if required, at which point the explants appear to be compl...

This research was designed to provide the first protocol establish an efficient solution for direct organogenesis regeneration in Passiflora quadrangularis using nodal explants from young shoots. Passifloraceae tissue culture has been associated with problems such as recalcitrance, sensitivity ethylene accumulation and browning of due presence phenols tissues. Due high rate endogenous contamina...