Polarity of the microtubule (MT) cytoskeleton is essential for many cell functions. Cytoplasmic linker-associated proteins (CLASPs) are MT-associated proteins thought to organize intracellular MTs and display a unique spatiotemporal regulation. In migrating epithelial cells, CLASPs track MT plus ends in the cell body but bind along MTs in the lamella. In this study, we demonstrate that glycogen...

Focal adhesions (FAs) control cell shape and motility, which are important processes that underlie a wide range of physiological functions. FA dynamics is regulated by cytoskeleton, motor proteins and small GTPases. Kinectin is an integral endoplasmic reticulum (ER) membrane protein that extends the ER along microtubules. Here, we investigated the influence of the ER on FA dynamics within the c...

The organs and tissues of insects are mostly composed of single layers of cells separated from the circulating blood by thin connective tissue membranes (Wigglesworth, 1956a; Pipa & Cook, 1958). The supply of nutrients to such organs presents no problem. But the central nervous system is an exception. The ganglia are the only solid organs in the insect body. They consist of densely packed perip...

The basement lamella under the epidermis of amphibian larvae shows a sub-microscopic architecture of remarkable geometric regularity: It consists of about twenty layers of ground substance in which cylindrical fibers (presumably collagenous) of about 500 Angström diameter are embedded parallel to one another, but with the fiber directions alternating by 90 degrees from layer to layer. The repai...

This paper concerns the drainage of a thin liquid lamella into a Plateau border. Many models for draining soap films assume that their gas-liquid interfaces are effectively immobile. Such models predict a phenomenon known as “marginal pinching”, in which the film tends to pinch in the margin between the lamella and the Plateau border. We analyse the opposite extreme, in which the gas-liquid int...

The direct contact of ultrathin polymer films with a solid substrate may result in thin film rupture caused by dewetting. With crystallisable polymers such as polyethyleneoxide (PEO), molecular self-assembly into partial ordered lamella structures is studied as an additional source of pattern formation. Morphological features in ultrathin PEO films (thickness < 10 nm) result from an interplay b...