The reaction between a5RuH2O2+ (a is NH3) and Pseudomonas aeruginosa azurin at pH 7, followed by oxidation, yields a5Ru(His-83)3+-azurin(Cu2+) as the major product. Spectroscopic measurements (UV-visible, CD, EPR, and resonance Raman) indicate that the native structure is maintained in the modified protein. The site of ruthenium binding (His-83) was identified by peptide mapping. The a5RuHis/Cu...

Cations have generally been reported to prevent jellyfish venom-induced hemolysis through multiple mechanisms by spectrophotometry. Little attention has been paid to the potential interaction between cations and hemoglobin, potentially influencing the antagonistic effect of cations. Here, we explored the effects of five reported cations, La3+, Mn2+, Zn2+, Cu2+ and Fe2+, on a hemolytic test syst...

The influence of SO2 presence during high temperature hydrothermal aging (600, 700, and 800 °C) on the performance Cu-SSZ-13 prepared by conventional ion-exchange method for selective catalytic reduction NOx NH3 (NH3-SCR) was studied. high-temperature activity is obviously enhanced 700 °C-aged samples not hampered 600 sample. has no framework Al. interaction Cu2+ with results in formation ZCuHS...

A sensitive and selective kinetic-spectrophotometric method is proposed for the determination of microg mL(-1) amounts of Cu2+ based on its catalytic effect on the oxidation of L-ascorbic acid by Methylene Blue in a strongly acidic medium. The reaction is monitored spectrophotometrically by measuring the decrease in color intensity of Methylene Blue at 665 nm. The analysis of Cu2+ ion is perfor...

Metformin in its protonated form as metformin hydrochloride is used worldwide an advanced antidiabetic drug for type 2 diabetes. Hyperinsulinemia and insulin resistance of cells are the main causes this disease. For patients with diabetes, (MET) works by improving cell sensitivity to insulin.
 It has been shown that methyl orange (MO) forms ionic associate can be isolated solid suitable cr...

A highly specific interaction between a metal-deficient metalloenzyme and metal ion has been utilized in the selective detection of the metal ion by surface plasmon resonance spectroscopy (SPRS). The use of SPRS and Cu-demetallated superoxide dismutase (E,Zn-SOD1) as a sensing actuator allows one to selectively and in situ detect Cu2+ without any interference that other spectroscopic methods ma...

Coated wire lithium ion selective electrode was fabricated using lithium ionophore-VI, 2-nitrophenyl octyl ether (NPOE) and potassium tetrakis(p-chlorophenyl)borate (KTpClPB) on platinum wire. Lithium ion selective electrode was giving Nernstian response from 10 -1 M to 10 -4 M with a detection limit of 3x10 -5 M. The calibration slope of this lithium ion selective electrode was found to be 58....

Conventional ion-selective electrodes with a liquid junction have the disadvantage of potential drift. All-solid-state ion-selective electrodes with solid contact in between the metal electrode and the ion-selective membrane offer high capacitance or conductance to enhance potential stability. Solution-casted chitosan/Prussian blue nanocomposite (ChPBN) was employed as the solid contact layer f...