An ultraviolet-laser microbeam was shown to be suitable for inducing fusion of individually selected plant protoplasts or of B-lymphocytes with myeloma cells. The fusion took place in normal culture medium and the fusogenic condition perturbed the cells only for a fraction of a millisecond. Without manipulating the cell culture except for exposing the cells to laser light, fusion products betwe...

background human enteroviruses are members of picornaviridae family; they are non-enveloped, icosahedral viruses with positive rna as genome. echovirus 30 is an important member of enteroviruses that is recognized in outbreaks of enterovirus meningitis. objectives the aim of this study was to determine relative frequency of echovirus 30 as an important agent of aseptic meningitis among children...

Mycobacterium leprae, despite being recognized as a human pathogen over 140 years ago, remains uncultivable in microbiological culture media or in cell culture systems. Although it is now well established that M. leprae prefers cooler temperatures, slightly acidic microaerophilic conditions, and lipids rather than sugars as an energy source, the exact parameters for a defined axenic medium that...

A sign&ant advance in the study of animal viruses was marked by the work of Schwerdt and Schaffer (l), who first obtamed purified poliovirus in quantities sufficient to permit rigorous chemical and physical characterization. Their procedure, however, required large volumes of starting material, and was both lengthy and laborious. The development of suspension culture techniques (2) has provided...

Human- and animal-pathogenic Bartonella species are fastidious and slow-growing bacteria difficult to isolate and cultivate. We describe a novel, easy-to-prepare liquid medium for the fast and reliable growth of several Bartonella spp. that does not affect bacterial protein expression patterns or interactions with host cells.

A method is described for subdividing S phase cells in asynchronous cell cultures on the basis of replication band patterns produced in chromosomes by bromodeoxyuridine incorporation. The criteria used for cell classification are objective, requiring the presence or absence of specific bands on particular chromosomes, and therefore lead to subdivisions amenable to quantitative analysis and for ...

Culture medium used in antibody purification contains growth factors, but is usually discarded after antibody purification. We examined to determine whether the growth factors can be (i) recovered and (ii) used in subsequent cell culture. Using this medium enhanced the survival of hybridoma cells and improved antibody production. Culture medium previously used in antibody purification offers an...

This article is a review highlighting the application of the acoustic filter as a reliable cell retention device during the long-term perfusion of animal cell cultures. Critical operating parameters such as duty cycle, perfusion and re-circulation flow rates, acoustic power and backflush frequency are discussed with regard to influence on the separation efficiency and optimal operating ranges h...

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied...