Mo/W formate dehydrogenases catalyze the reversible reduction of CO2 species to formate. It is thought that substrate and not a hydrated like HCO3?, but there still no indisputable evidence for this, in spite extreme importance nature mechanistic studies. We devised simple electrochemical method definitively demonstrate indeed CO2.

It has previously been shown that cell-free extracts of Acetobacter subozydans possess two pathways for the oxidation of sorbitol (1). In the presence of DPN,l fructose is produced; with TPN, sorbose is formed. The primary purpose of this paper is to describe the separation of these two dehydrogenases from each other. The DPN enzyme free from TPN activity has been concentrated about lBfold, whe...

Alcohol dehydrogenases (ADHs) have become important catalysts for stereoselective oxidation and reduction reactions of alcohols, aldehydes ketones. The aim this contribution is to provide the reader with a timely update on state-of-the-art ADH-catalysis. Mechanistic basics are presented together practical information about use ADHs. Current concepts ADH engineering critically discussed. Finally...

NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate conversion was not impaired in these strains, while it was completely blocked in ...

is catalyzed by glutathione reductase (3, 4, 5). In earlier studies this reaction has been coupled with soluble dehydrogenases which utilize TPN as a coenzyme (6). Since tissue fractionation studies have shown that numerous dehydrogenases, including those associated with the oxidation of the Krebs cycle intermediates, are localized within the mitochondrion, it was of interest to determine wheth...

The present studies are concerned with the determination of the steric course of the transfer of hydrogen catalyzed by hydroxysteroid dehydrogenases. The classic work of Vennesland et al. (l-3) has demonstrated that pyridine nucleotide-linked dehydrogenases catalyze a direct and stereospecific transfer of hydrogen between the substrate and the para position of the nicotinamide ring of the nucle...