to determine the extent and the size of the environment pollution with heavy metals, biologicalmethods, investigating the enzymatic activity of soil can be applied. most frequently, the enzymes investigated in the soils threatened with anthropogenic contamination are phosphatases, dehydrogenases as reacting fastest to the increase in the content of heavy metals in the environment. the aim of th...

T h e structure, function and evolution of alcohol dehydrogenases and aldehyde dehydrogenases have been studied for several years. Recent progress o n these aspects has been considerable and allows extensive conclusions to be drawn about the relationships and properties of these enzymes. Further aspects have also become available for study, such as the regulation of enzyme expression and the an...

The capacities of Procion Red HE-3B and Cibacron Blue F3G-A immobilized to Sepharose CL-4B and Matrex 201R for NAD+-, NADP+- and NAD(P)+-dependent dehydrogenases were measured. Procion Red HE-3B columns retarded NADP+-dependent dehydrogenases more effectively than NAD+-dependent dehydrogenases, whilst immobilized Cibacron Blue F3G-A retarded NAD+-dependent dehydrogenases more effectively than N...

The N-terminal sequences of malate dehydrogenases from 10 bacterial strains, representing seven genera of Proteobacteria, were determined. Of these, the enzyme sequences of species classified in the genus Brevundimonas clearly resembled those malate dehydrogenases with greatest similarity to lactate dehydrogenases. Additional evidence from subunit molecular weights, peptide mapping, and enzyme ...

Vertebrate and invertebrate L-lactate dehydrogenases (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) are effectively bound to oxamate-diaminohexyl-Sepharose, whereas several D-lactate dehydrogenases (D-lactate:NAD+ oxidoreductase, EC 1.1.1.28) do not bind to the same Sepharose. One explanation for our findings is that the enzymes' substrate is oriented in a reversed manner in the active center of ...

to determine the extent and the size of the environment pollution with heavy metals, biologicalmethods, investigating the enzymatic activity of soil can be applied. most frequently, the enzymes investigated in the soils threatened with anthropogenic contamination are phosphatases, dehydrogenases as reacting fastest to the increase in the content of heavy metals in the environment. the aim of th...

1. The reversible dissociation of lactic and malic dehydrogenases has been studied in detail and compared with the dissociation properties of triosephosphate dehydrogenase, a-glycerophosphate dehydrogenase, and liver alcohol dehydrogenase. 2. Evidence is presented for the existence of an intermediate form of lactic dehydrogenase with catalytic properties altered during reactivation. 3. The tech...

Abstract Metal‐based formate dehydrogenases are molybdenum or tungsten‐dependent enzymes that catalyze the interconversion between and CO 2 . According to current consensus, metal ion of catalytic center in its active form is coordinated by 6 S (or 5 1 Se) atoms, leaving no free coordination sites which could bind metal. Some authors have proposed one site ligands decoordinates during turnover ...

Stereospecificity of Some Invertebrate Lactic Dehydrogenases by Mary Jacobs Scheid 1. It has been assumed that only among unicellular organisms did nicotin¬ amide adenine dinucleotide (NAD)-linked lactic dehydrogenases occur. All higher animals were thought to possess Lspecific lactic dehydro¬ genases. It was discovered recently, however, that many species of invertebrates possess lactic dehydr...