Methods enabling mutational analysis of distinct chromosomal locations, like site-directed mutagenesis, insertion of foreign sequences or in-frame deletions, have become of fast growing interest since complete bacterial genome sequences became available. Various approaches have been described to modify any nucleotide(s) in almost any manner. Some genetic engineering technologies do not rely on ...

zymatic and biochemical propenies were examined. Three mutallts, P50S, DI08L, and DIZ3N, showed fairly low Hindlll activity. Oll the ether halld, the other £ouv, P84Q, E86K, VI06E, alld K125N, retained the activity. In particu1ar, E86K showed higher actiyity than the wild enzyme. This fact was confirmed when actiyities ef the purMed wild and E86K enzymes were assayed. These results coincided fa...

prolactin is mainly secreted by the anterior pituitary and is able to stimulate mammary gland development and lactation in mammalians. although prolactins share a common ancestral gene encoding, they show species specific characteristics and their efficiency may be different in various mammals. the importance of protein structures of all sequences of this hormone have been studied by various bi...

Prolactin is mainly secreted by the anterior pituitary and is able to stimulate mammary gland development and lactation in mammalians. Although prolactins share a common ancestral gene encoding, they show species specific characteristics and their efficiency may be different in various mammals. The importance of protein structures of all sequences of this hormone have been studied by various bi...

     The aim of this study was to use site directed mutagenesis technique to construct a vector in which serine363 and serine375 residues of the COOH-terminal portion of the μ-opioid receptor (MOR) were substituted by alanine. These constructs are essential in studying G-protein coupled receptor kinase-mediated MOR desensiti-zation. The nested PCR carried out for conversio...