OBJECTIVE To evaluate DNA fragmentation in spermatozoa with normal morphological appearance. DESIGN Prospective study. SETTING Academic tertiary center. PATIENT(S) Fertile, subfertile, and infertile men were studied. INTERVENTION(S) Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-fluorescein nick-end labeling assay and morphology assessment by phase contrast in ...

Background: Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa. Also, decreased telomere length in spermatozoa of infertile men is associated with the reduction of fertility potential and embryo quality. Density gradient centrifugation (DGC)...

Rheum turkestanicum Janischew. (Polygonaceae) is a plant that grows in central Asia and in north-east of Iran. Traditionally, people use roots of R. turkestanicum as an anti-diabetic and anti-hypertensive as well as anticancer agent. In this study the cytotoxicity and apoptogenic properties of ethyl acetate (EtOAc), n-hexane and H2O extracts from Rheum turkestanicum Janischew. (Polygonaceae) ro...

Background: Varicocele is considered as a common cause of male infertility and can affect spermatogenesis. In addition, it also predisposed sperm to chromatin damage and morphological alternation. Other factors, such as environmental and genetic conditions also predispose sperm to these conditions. To answer this question, we aimed to evaluation of DNA fragmentation and protamine deficiency thr...

Objective. To investigate sperm DNA fragmentation and sperm functional maturity in men from infertile couples (IC) and men with testicular germ cell tumor (TGCT). Materials and Methods. Semen samples were collected from 312 IC men and 23 men with TGCT before unilateral orchiectomy and oncological treatment. The sperm chromatin dispersion test was performed to determine DNA fragmentation index (...

The objective of the presented study was determination of the effect of selected agents on sperm DNA fragmentation--superoxide dismutase in seminal plasma, the patients' age, and burdening with the tobacco smoking habit. An attempt was also undertaken to evaluate the effect of DNA fragmentation on the effectiveness of infertility treatment. The study covered 186 men who received treatment due t...

Following the sexual cycle, hypotrichous ciliated protozoans fragment a set of their micronuclear chromosomes to generate the thousands of short, linear DNA molecules present in the transcriptionally active macronucleus. We have used a hybrid selection procedure to examine macronuclear DNA molecules for subtelomeric length heterogeneity to determine whether chromosome fragmentation occurs at un...

varicocele is one of the most common causes of primary male infertility. pilea microphylla (pm) is being used as folk medicine. this study was aimed to investigate the effects of pm in a rat model of varicocele. a total of 30 male wistar rats were divided into control, sham, varicocele, accessory varicocele and pm-treated groups. after 10 weeks of varicocele induction, sperm parameters and chro...

Background: Globozoospermia is an uncommon disorder of sperm morphology associated with severe male infertility. Patients who contain both normal and round-headed sperm are called partial globozoospermic. It is possible that round-headed sperm may have an abnormal chromatin structure and /or DNA strand breaks. Moreover, sperm DNA fragmentation and protamine deficiency is shown to be associated ...