BACKGROUND The present study was undertaken in order to establish phenotypically different cell clones from 10 parental lines of human breast (MCF-7 and T-47D), prostate (PC-3 and DU145), lung (A549 and A427), colon (HCT-116 and HT-29) and bladder (TCCSUP and T24) cancer cells. MATERIALS AND METHODS Sublines were established from each of the parental lines by the limiting dilution method. The...

Background . Melittin is a major constituent of honeybee venom and comprises water-soluble surfactant peptide with cytolytic effects potentially applicable in anticancer therapy. We evaluated the impact melittin from Bashkir ( Apis mellifera L.) on cell viability various prostate cancer lineages. Materials methods. MTT assays index estimation were used to evaluate effect proliferation various-g...

background: most cancer cells become resistant to anti-cancer agents. in the last few years, a new approach for targeted therapy of human cancer has been developed using immunotoxins which comprise both the cell targeting and the cell killing moieties. methods: in the present study, the recombinant shiga toxin a1 subunit fused to human granulocyte-macrophage colony stimulating factor (a1-gm-csf...

in this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in iran. the cells were originating from two-day-old normal male calf of holstein breed. the cell cultures were continuously passaged following complete proliferation of primary cells. the specific properties or characteristics of the cell were defined using cytogene...

Abstract Intracellularly active complement C3 and C3a emerged as central regulators of normal immune cell function proliferation via control mTOR glycolysis. Because increased glycolysis is a hallmark many cancer cells, we hypothesized that augmented cell-intrinsic activity may contribute to epithelial malignancy. However, while benign prostate cells (PECs) harbored expected intracellular C3/C3...

Prostate cancer (PCa) is a leading cause for death in men and the most commonly diagnosed malignancy globally. MicroRNA (miR)‑583 expression levels have been discovered to be downregulated recurrent PCa samples compared with non‑recurrent cases. However, precise functions pathogenic mechanism of miR‑583 development are vague, thus aim present study was investigate these. The Janus kinase 1 (JAK...

Present: Due to incorrect labeling on the part of the authors, it was discovered that a different set of cell lines was used than those initially published. The authors confirmed that all the research data presented in this paper were based on in vitro studies with human CRPC DU145 cells, and not with human CRPC PC3 cells. Therefore, the correct title for this paper should be: “MtDNA depleted D...

Many prostate cancers relapse due to the generation of chemoresistance rendering first-line treatment drugs like paclitaxel (PTX) ineffective. The present study aims to determine the role of miRNAs and Hedgehog (Hh) pathway in chemoresistant prostate cancer and to evaluate the combination therapy using Hh inhibitor cyclopamine (CYA). Studies were conducted on PTX resistant DU145-TXR and PC3-TXR...

Eight lichens were extracted successively with n-hexane, diethyl ether and methanol using a Soxhlet process. The cytotoxic activity of the 24 lichen extracts was evaluated in vitro using two murine (the L1210: lymphocytic leukaemia, and the 3LL: Lewis lung carcinoma) and four human (the K-562: chronic myelogenous leukaemia, the U251: glioblastoma, the DU145: prostate carcinoma, and the MCF7: br...

Halophilic archaea are known as the novel producers of natural products and their supernatant metabolites could have cytotoxic effects on cancer cells. In the present study, we screened the anticancer potential of supernatant metabolites from eight native haloarchaeal strains obtained from a culture collection in Iran. Five human cancer cell lines including breast, lung, prostate and also human...