This paper describes enzyme-linked immunosorbent assays (ELISA) performed in a 96-microzone plate fabricated in paper (paper-based ELISA, or P-ELISA). ELISA is widely used in biochemical analyses; these assays are typically carried out in microtiter plates or small vials. ELISA combines the specificity of antibodies with high-turnover catalysis by enzymes to provide specificity and sensitivity....

Several models of authorization have been proposed for object-oriented databases supporting diierent levels of granularity. However, these models do not support authorization based on database contents and context. A way of handling context and content-dependent authorization is by using views. In this paper, we present a model of authorization, based on a view model proposed by Bertino 4], tha...

objective: helicobacter pylori is to blame for one of the most common chronic infections in humans and can cause peptic ulcer, gastritis, duodenitis and non-ulcer dyspepsia. developing a reliable test to detect the infection is of great importance. for a variety of reasons, the existing tests including the stool culture, biopsy, pcr, and urea breath test are not up to par. the pcr-elisa test is...

background and aims: the aim of this study was application of gamma irradiated fmdv antigen for liquid phase blocking sandwich elisa (b-elisa) to quantify guinea pig anti fmdv type a87/irn antibody. methods: fmdv type a87/irn was propagated to a titer of 107.5/ml tcid50 and then irradiated by gamma irradiator. the irradiated 146 s antigen was purified by sucrose gradient centrifugation after in...

in order to study the trend of bnyvv concentrations during growing season, 6 cultivars including dorohtea, laetitia(as resistant cultivars), zarghan (as a tolerant cultivar), shirin (as a susceptible cultivar), f2-93 (an f2 population with 75 % rz2 gene) and bc1-261-99 (a population with 25% rz2 gene) were used. the cultivars were planted in split plot based on a randomized complete block desig...

ABSTRACT: Bovine viral diarrhea virus (BVDV) is a highly infectious pathogen that affects bovines worldwide leading to great economic impact. Although Brazil has the largest commercial cattle population throughout world and an increasing buffalo breeding industry, country no control or eradication program for BVDV. In this perspective, aim of study was evaluate occurrence BVDV in buffaloes from...

The results of a field survey some potato growing areas in Nineveh Governorate showed the presence Potato virus s PVS, which was detected by Double Antibody Sandwich Enzyme-Linked ImmunoSorbent Assay DAS-ELISA test. This record is first governorate. Through absorbance readings using elisa reader, highest value recorded Sherikhan area samples, amounted to 1.190 nm, while lowest Rabia samples wit...