we describe a rapid, simple, and reliable procedure for routine isolation of mycobacterium leprae dna from slit-skin swab specimens. this one-step dna extraction method is based on the utilization of chelexr 100, a chelating ion exchange resin. slit-skin swab specimens from seven untreated leprosy patients at baba baghi leprosy hospital in iran were processed by this procedure. the polymerase c...

The coupling between the presence of predominant bacterial species and particular biogeochemical processes is of primary interest in current microbial ecology. Molecular methods such as microarrays and real-time polymerase chain reaction (PCR) may be used to estimate presence or expression of different genes (e.g., 16S rDNA and nifH) in environmental samples; however, to be quantitative, these ...

A microwave-assisted extraction approach based on ionic liquids of different chain lengths was successfully applied to the extraction of ten flavonoid glycosides from the flowering heads of Chrysanthemum morifolium Ramat. The pretreated sample was quantified by HPLC-ESI-MSn. The main components were identified as flavonoid glycosides, including three luteolin glycosides, three apigenin glycosid...

The fruit of Melastoma sanguineum Sims is an edible and sweet wild fruit. In our previous study, the fruit was found to have a strong antioxidant property. In this study, an ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the fruit of Melastoma sanguineum Sims, and a response surface methodology was used to optimize the conditions of UAE to maximiz...

Automatic facial feature extraction is one of the most important and attempted problems in computer vision. It is a necessary step in face recognition, facial image compression. There are many methods have been proposed in the literature for the facial feature extraction task. However, all of them have still disadvantage such as not complete reflection about face structure, face texture. Theref...

INTRODUCTION The aim of this retrospective study was to compare the occlusal outcomes, duration and efficiency of Class I malocclusion treatment carried out with and without premolar extractions in patients with different degrees of initial malocclusion severity. METHODS Complete records of 111 patients were obtained and divided into two groups: Group 1 consisted of 65 patients at an initial ...

Light extraction efficiency of light-emitting diodes (LEDs) based on various photonic crystal (PhC) structures is investigated in this study. By using the plane wave method and the finite element method, the influence of several factors on the enhancement of light extraction is discussed, including lattice type, the density of states from a photonic band diagram, the ratio of cylinder radius an...

We represent an environmentally friendly sample of pre-treatment method, ultrasound assisted emulsification microextraction (USAEME) followed by gas chromatography nitrogen phosphorous detector (GC-NPD), to specify atrazine residues in environmental samples. Some parameters affecting the extraction efficiency such as the type and volume of the extraction solvent, emulsification time and additio...

Background and Objectives: Heterocyclic aromatic amines (HAAs) are carcinogenic and mutagenic substances, which are formed during the heating process of proteinaceous foods such as meat and meat products. The extraction of these compounds from the complex matrix of meat products was carried out by microwave assisted pre-extraction and a precise, rapid and high sensitive microextraction method. ...