stochastic approach to vehicle routing problem: development and theories abstract in this article, a chance constrained (ccp) formulation of the vehicle routing problem (vrp) is proposed. the reality is that once we convert some special form of probabilistic constraint into their equivalent deterministic form then a nonlinear constraint generates. knowing that reliable computer software for lar...

Tumor growth after radiotherapy is a commonly recognized cause of therapeutic failure. In this way, we examined tumor cell growth after radiotherapy by establishing a cancer cell growth model in vitro. We accomplished this model by seeding non-irradiated firefly luciferase2 and green fluorescent protein fusion gene (Fluc) labeled living cancer reporter cells onto a feeder layer of irradiated ca...

In order for human embryonic stem cells (hESCs) to be cultured on mouse embryonic fibroblast (MEFs) feeder cells, continuous basic fibroblast growth factor (bFGF) supplementation is required. However, the role of bFGF in a culture system using human-derived feeder cells has not been evaluated until now. In this study, we propagated the widely used hESC lines, H1 and HSF6, on human placenta-deri...

The feeder layer constitutes a prerequisite for the undifferentiated proliferation of human embryonic stem (hES) cells in vitro. However, a few feeders have been reported to be nonsupportive in nature, suggesting that these feeders exhibit a different transcriptome and proteome, in comparison to their supportive counterparts. In an attempt to identify factors required for undifferentiated growt...

Umbilical cord blood (UCB) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (HSCs). Because of insufficient number of cord blood CD34(+) cells, the expansion of these cells seems to be important for clinical application. Mesenchymal stromal cells (MSCs), playing an important role in HSCs maintenance, were used as feeder layer. A...

BACKGROUND For therapeutic usage of induced Pluripotent Stem (iPS) cells, to accomplish xeno-free culture is critical. Previous reports have shown that human embryonic stem (ES) cells can be maintained in feeder-free condition. However, absence of feeder cells can be a hostile environment for pluripotent cells and often results in karyotype abnormalities. Instead of animal feeders, human fibrob...