Treating solid tumours with chimeric antigen receptors-modified T cells (CAR-T cells) has shown limited efficacy due to the lack of cancer-type specific antigens. Dinutuximab is a monoclonal antibody that recognizes sphingolipid, disialoganglioside GD2, which expression in normal tissues but overexpressed paediatric tumours, mainly neuroblastoma (NB) and diffuse intrinsic pontine glioma (DIPG) ...

BACKGROUND We developed a single-color multitarget flow cytometry (SM-FC) assay, a single-tube assay with graded mean fluorescence intensities (MFIs). We evaluated the repeatability of SM-FC, and its correlation with multicolor flow cytometry (MFC), to assess its application as a routine FC assay. METHODS We selected CD19, CD3, CD4, and CD8 as antigen targets to analyze a lymphocyte subset. M...

The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects but, uptake these nanocarriers induce adverse on cells tissues after their incorporation. Macrophages play a central role in host defense are responsible vivo nanoparticle trafficking. Assessment capacity against pathogenic micro-organisms uptake, is necessary to prevent infections associated wit...

Na+,K+-ATPase from pig kidney was sequentially modified with N-[p-(2-benzimidazolyl)phenyl]maleimide (BIPM) at Cys-964 and fluorescein isothiocyanate (FITC) at Lys-501. The resulting preparation showed little Na+,K+-ATPase activity with retention of nearly 90% of phosphorylation capacity from acetyl phosphate. The addition of acetyl phosphate to the preparation induced phosphoenzyme formation w...

Due to health concerns about phthalate esters, the use of alternative plasticizers is being considered. Phthalate esters enhance skin sensitization to fluorescein isothiocyanate (FITC) in mouse models. We have demonstrated that phthalate esters stimulate transient receptor potential ankyrin 1 (TRPA1) cation channels expressed on sensory neurons. We also found a correlation between TRPA1 activat...

BACKGROUND Detection of apoptosis in sperm samples may help evaluate sperm quality. Recently, it has been suggested that in some ejaculated sperm populations, apoptosis is caspase dependent. The aim of this study was to investigate the presence of activated caspases and examine possible correlations with apoptosis and sperm parameters in semen samples prepared for IVF. METHODS To detect activ...

In gene therapy, how genetic therapeutics can be efficiently and safely delivered into target tissues/cells remains a major obstacle to overcome. To address this issue, nanoparticles consisting of non-covalently coupled polyethyleneimine (PEI) and folic acid (FA) to the magnetic and fluorescent core/shell of Fe3O4@SiO2(FITC) was tested for their ability to deliver Notch-1 shRNA. Our results sho...

Peroxidase-conjugated antibodies were found to be as sensitive as those conjugated to fluorescein-isothiocyanate (FITC) for identification of selected enterovirus types by the indirect technique. Peroxidase conjugates, however, were found to give fewer nonspecific reactions. The reasons for the higher specificity of the immunoperoxidase technique appear to be related to the relative size, charg...

Phage display technology has been demonstrated to be a powerful tool for screening useful ligands that are capable of specifically binding to biomarkers on the surface of tumor cells. The ligands found by this technique, such as peptides, have been successfully applied in the fields of early cancer diagnostics and chemotherapy. In this study, a novel nonsmall cell lung cancer-targeting peptide ...