We present an efficient generalization of the sparse pseudo-input Gaussian process (SPGP) model developed by Snelson and Ghahramani [1], applying it to binary classification problems. By taking advantage of the SPGP prior covariance structure, we derive a numerically stable algorithm with O(NM) training complexity—asymptotically the same as related sparse methods such as the informative vector ...

We present an efficient generalization of the sparse pseudo-input Gaussian process (SPGP) model developed by Snelson and Ghahramani [1], applying it to binary classification problems. By taking advantage of the SPGP prior covariance structure, we derive a numerically stable algorithm with O(NM) training complexity—asymptotically the same as related sparse methods such as the informative vector ...

This study examines leukocytes present in lymphoedema (LE) adipose tissue (AT) by multi-colour confocal microscopy. LE AT, collected liposuction surgery, was digested with collagenase to separate adipocytes from other cells, comprising blood and lymphatic endothelial fibroblasts, all vessel- tissue-resident - the stromal vascular fraction (SVF). SVF cells were activated phorbol 12-myristate 13-...

Here we describe the preparation of BSA-FITC-loaded microcapsules as a model protein system for in vivo delivery. BSA-FITC-loaded microcapsules were prepared using a mono-axial nozzle ultrasonic atomizer, varying a number of parameters to determine optimal conditions. The preparation method chosen resulted in a BSA-FITC encapsulation efficiency of approximately 60% and a particle size of approx...

This study sought to evaluate FITC-conjugated cyclic RGD peptides (FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2) as fluorescent probes for in vitro assays of integrin αvβ3/αvβ5 expression in tumor tissues. FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2 were prepared, and their integrin αvβ3/αvβ5 binding affinity was determined using the displacement assay against (125)I-echistatin bound to U8...

PURPOSE To develop an adaptable gene-based vector that will confer immune cell specificity to various cancer types. EXPERIMENTAL DESIGN Human and mouse T cells were genetically engineered to express a chimeric antigen receptor (CAR) that binds a fluorescein isothiocyanate (FITC) molecule, termed anti-FITC CAR T cells. Various antibodies (Ab) currently in clinical use including cetuximab (Ctx)...