Rates of lipolysis and reesterification were determined under various conditions in adipocytes from epididymal fat pads of old, spontaneously obese rats and compared to cells from younger, leaner animals. No differences were observed in lipolytic responsiveness to several concentrations (2--50 microM) of the beta-adrenergic agent ritodrine compared to cells from younger, leaner rats. The large ...

A decreased capacity for fatty acid synthesis is associated with a decreased insulin effect on glucose metabolism in large fat cells and fat cells from rats fed a high-fat diet. We have investigated the relationship between these processes by specifically inhibiting fatty acid synthesis with (-)-hydroxycitrate (2.5 mM), an inhibitor of citrate cleavage enzyme, and cerulenin (0.05 mM), an inhibi...

A number of studies (l-8) have attempted to identify the digestion products of glycerides that are absorbed from the lumen of the intestine. Unfortunately none of these unequivocally established the nature of the absorbed materials. I f they had, they would have revealed also the extent of rearrangement of the fatty acids of the dietary triglycerides. Moreover, because of the type of materials ...

Isolated fat cells were used for the study of in vitro effects of insulin on glucose metabolism in human and rat adipose tissue. In human subcutaneous fat cells, effects of insulin could be detected at concentrations of glucose in the medium from 1 to 10 micro moles/ml. Cellular responsiveness was inversely proportional to the glucose level. At a constant concentration of 6 micro moles of gluco...