The behavior of human omental fat cells in vitro has been examined in order to define conditions under which glucose is converted to glyceride-glycerol and glyceride fatty acids. Synthesis of glyceride fatty acids from glucose reached maximal rates only after several hours of incubation in Krebs-Henseleit bicarbonate buffer, with or without added bovine albumin. Conversion of glucose to glyceri...

The metabolism of propionate in adipose tissue and its effect on lipogenesis was investigated. Fasting induced changes in propionate metabolism of adipose tissue, drastically reducing higher fatty acid synthesis and increasing glyceride-glyerol formation from low concentrations of propionate (0.25 mM). Propionate also promoted lipogenesis from acetate-1-(14)C in tissues of fasted rats, while it...

[1-(14)C]glucose oxidation to CO(2) and conversion into glyceride by adipose tissue from nonobese and obese subjects has been studied in vitro in the presence of varying medium glucose and insulin concentrations as functions of adipose cell size, the composition of the diet, and antecedent weight gain or loss. Increasing medium glucose concentrations enhance the incorporation of glucose carbons...

Lipid metabolism is a growing area of biochemical research because understanding these pathways could lead to treatments for metabolic disorders such as obesity and type 2 diabetes. To study lipid metabolism, researchers need tools to identify and quantitate glycerides, the main component of animal fat. However, it can be difficult to tell one glyceride apart from another subtly different glyce...

Various aspects of glycerol metabolism have been extensively investigated, both in vivo and in vitro, with the aid of isotopic glycerol. Experiments in vivo on the incorporation of C14-glycerol into glycogen and various body lipides, as well as its oxidation to C1402 have been reported (l-3). The metabolism of Ci4-glycerol by liver in vitro (4) and glycogen synthesis from deuterium-labeled glyc...

This work describes the fatty acid and glyceride structures of Indian buffalo milk and depot fats. Only three other an4lyses of buffalo milk fat exist in the literature (Bhattacharya & Hilditch, 1931; Heiduschka & Cicekdagi, 1940), in only one of which was an examination made of the glyceride configuration, while no analysis of buffalo depot fat has so far been reported. The work below was ther...

Role of the liver in glyceride metabolism In mammals, there are three major forms of circulating lipid or lipid-related fuel, free fatty acid (FFA), triglyceride (TG) and ketone bodies. The liver utilizes FFA, and releases T G and ketone bodies. Constituents of the pool of carbohydrate plus amino acids can undergo net conversion to acetyl residues and then to fatty acids (FA) (FA synthesis ‘de ...

The rate and pattern of glucose metabolism, basal lipolysis, and intracellular concentration of free fatty acids were determined in isolated epididymal fat cell preparations (mean volume 30-800 pl) from rats on the basis of fat cell number and in relation to the cell volume. The effects of increasing glucose concentrations in the medium and of insulin on the cellular metabolic activities were c...