A new method of fabricating electrodes for microchip devices that involves the use of Teflon molds and a commercially available epoxy to embed electrodes of various sizes and compositions is described. The resulting epoxy base can be polished to generate a fresh electrode and sealed against poly(dimethylsiloxane) (PDMS)-based fluidic structures. Microchip-based flow injection analysis was used ...

Nowaday, the construction of new electrochemical biosensors with improved bioanalytical performance and robustness is directly linked to the development of novel strategies for tailormade design of electrode surfaces. Such strategies should provide electrical interfaces with nanoor microsized tree-dimensional topology that allow the successful and stable immobilisation of the biomolecules witho...

We explored the effect of an applied electric field on molecular conformation two weak polyelectrolytes grafted gold electrodes?poly(acrylic acid) and polybase poly(2-(dimethylamino)ethyl methacrylate)?by combining a quartz crystal microbalance with electrochemical setup. Our results indicate that different mechanisms influence response to externally potential difference, including modification...

In the present study, a new biosensor for 5-Fluorouracil was described using modified goldelectrode and Fast Fourier transform square wave voltammetry (FFT SWV). Calf thymus DNAimmobilization was on a gold electrode decorated with polyaniline and gold nanoparticles. Theelectrochemical characteristics of the electrodes were investigated by cyclic voltammetry, andelectroch...

Isoniazid (INH) was studied with regard to its electrochemical treatment on a strongly alkaline solution of a poly (3,4-ethylenedioxythiophene)-modified gold electrode, using both cyclic voltammetry and controlled potential techniques. This composite electrode exhibited 4-fold higher oxidation of INH, in accordance with peak densities, than the bare gold electrode. It was used the central compo...

An electrochemical affinity sensor for the determination of fumonisins mycotoxins (Fms) using monoclonal antibody modified screen-printed gold electrode with carbon counter and silver-silver chloride pseudo-reference electrode is reported in this work. A direct competitive enzyme-linked immunosorbent assay (ELISA) was initially developed, exhibiting a detection limit of 100 µg·L(-1 )for fumonis...

An electrochemical DNA biosensor for the detection of NOS gene sequences from genetically modified organisms (GMOs) is presented in this paper. Single-stranded DNA (ssDNA) was covalently attached through the carboxylate ester formed by the 3'- hydroxy end of the DNA with the carboxyl of a mercaptoacetic acid self-assembled monolayer-modified gold electrode using N-hydroxysuccinimide (NHS) and N...

An in-situ electrochemical Rutherford backscattering (ECRBS) system has been demonstrated that enables the investigation of electrode and solution composition, along with kinetics of nuclide adsorption and desorption, at an electrode-electrolyte interface (EEI) under potential control. In contrast to other in-situ electrochemical diffraction or spectroscopy techniques, ECRBS can provide quantit...

Electrochemical oxidation of quercetin, as an important biological molecule, has been studied in non-aqueous media using cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. To investigate the electrochemical properties of quercetin, an important flavonoid derivative, on a different surface, a new glassy carbon electrode has been developed using dithiooxa...