We report a family with a new hexokinase variant that gives rise to nonspherocytic hemolytic anemia in one apparently homozygous family member. The variant enzyme has a normal pH optimum, normal reaction kinetics, and normal electrophoretic properties, but has reduced activity and is apparently inactivated rapidly as the affected erythrocytes age.

Introduction: Drug-induced immune hemolytic anemia (DIIHA) is known as an unpredictable and in some cases serious complication occurring upon pharmacotherapy. Cefazolin, as a first generation cephalosporin used against gram-negative bacteria, has rarely been reported to cause hemolytic anemia. Here, we report the first fatal case of hemolytic anemia associated with cefazolin treatment. Material...

The hemolytic activities of 91 strains of Vibrio parahaemolyticus isolated from human diarrheal stools, sea fish, and sea water; 21 suspected V. parahaemolyticus cultures isolated from wound infections; 14 nonpathogenic marine vibrios; and 21 V. parahaemolyticus isolated from moribund blue crabs Callinectes sapidus were compared. Potentially pathogenic V. parahaemolyticus strains could be diffe...

The ƒ¿-toxin of Clostridium perfringens (werchii) contains both the lethal and hemolytic activities. It was identified as phospholipaseC (EC 3.1.4.3) catalyzing the hydrolysis of lecithin to a diglyceride and phosphorylcholine (Macfarlane and Knight, 1941). Pastan et al. (1968) separated the phospholipaseC of C. perfringens into two fractions, one preferentially hydrolyzing sphingomyelin to cer...

objective(s): streptococcus pyogenes produces extracellular hyaluronidase enzyme. this enzyme is directly associated with the spread of the organism during infection. the objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. materials and methods: the enzymatic region of hyaluronidase gene was detecte...

Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) technique to detect the gene for aerolysin in strains of Aeromonas hydrophila and to screen for identical genes in A. caviae, A. sobria, and A. veronii isolated from patients with diarrheal disease. Primers targeted a 209-bp fragment of the aer gene coding for the beta-hemolysin and detected template DNA only in th...

Several hemolytic markers are available to guide the differential diagnosis and to monitor treatment of hemolytic conditions. They include increased reticulocytes, an indicator of marrow compensatory response, elevated lactate dehydrogenase, a marker of intravascular hemolysis, reduced haptoglobin, and unconjugated hyperbilirubinemia. The direct antiglobulin test is the cornerstone of autoimmun...

M ICROAN(;IOPATHIC HEMOLYTIC ANEMIA in humans complicates a variety of vascular diseases. This hemolytic process is characterized by fragmentation of erythrocytes with the production of irregularly contracted erythrocytes ( including “burr” cells, “helmet” cells, and schistocytes ) and spherocytes. The condition may develop in thrombotic thrombocytopenic purpura, malignant hypertensiomi, bilate...

The study of immune hemolytic anemias has contributed significantly to the understanding of the immune response in the human system in the past. Thus, the concept of receptor specificity – becoming the rationale of the clonal selection theory when trying to explain how the organism avoids autoaggression – has been developed originally upon the study of immune hemolytic anemias, and the question...