PURPOSE OF REVIEW One-lung ventilation in the thoracic surgical patient can be achieved with the use of a double-lumen endotracheal tube or an independent bronchial blocker. A number of patients requiring lung isolation have a potentially difficult airway because of previous radiation to the neck or previous surgery to the tongue and larynx. This review will focus on the management of patients ...

Modern websites and web applications commonly integrate third-party and user-generated content to enrich their users’ experience. Developers of these applications are in need of a simple way to limit the capabilities of this less trusted, outsourced content and thereby protect their users from cross-site scripting attacks. We summarize several recent proposals that enable developers to isolate ...

Since the 1960s, several new attempts have been made to improve the management of single prokaryotic cells using micromanipulator techniques. In order to facilitate the isolation of pure cultures we have recently developed an improved micromanipulation method for routine work. With the aid of this method single prokaryotic cells can be picked out of a mixed community under direct visual control...

UNLABELLED  Background. The following describes successful isolation of enteroatmospheric fistulae within a negative pressure wound therapy system (V.A.C.®, KCI, San Antonio, TX). METHODS An impermeable tubular structure was placed around the fistula with a dressing applied to the surrounding wound base, dressed with an impermeable drape and negative pressure, and then an ostomy applianc...

The SP-4 culture medium, developed originally for newly isolated plant and insect mycoplasmas (spiroplasmas), has markedly improved the recovery of Mycoplasma pneumoniae from human clinical materials. This medium, in combination with a direct fluorescent antibody test, can enhance the recovery and identification of the organism by 30-40 percent over conventional culture procedures. Although the...

Despite the recent interest in viruses infecting eucaryotic marine microalgae, only 7 viruses infecting marine phytoplankton have been isolated so far. It is therefore worth reviewing isolation techniques and rationalizing the method for obtaining novel microalgal viruses. I consider 7 steps as critical in the isolation procedure: (1) selection of the target host alga, (2) preparation of target...

Magnetic separation is an emerging technology using magnetism, sometimes in combination with conventional separation or identification methods, to purify cells, cell organelles and biologically active compounds (nucleic acids, proteins, xenobiotics) directly from crude samples. Several magnetic separation procedures have been developed to isolate target cells specifically. The purpose of this s...

Isolation of Japanese encephalitis (JE) virus using C6/36 cell and immunofluorescence virus antigen detection techniques was attempted from female mosquitoes collected with CDC gravid traps in Samut Songkhram Province in the central region and in Phuket Province in southern Thailand, in 2003. One thousand and eighty female mosquitoes including 6 species of the Culicidae family (Culex quinquefas...