Modern techniques of laser microsurgery of cell spheroids were used to develop a new simple reproducible model for studying repair and regeneration in vitro Nanosecond laser pulses (wavelength 355 nm, frequency 100 Hz, pulse duration 2 ns) were applied to perform a microdissection of the outer and the inner zones of human bone marrow multipotent mesenchymal stromal cells (BM MMSC) spheroids. To...

AIM Curative treatment options for laryngeal carcinoma include primary radiation therapy, open surgical techniques and transoral laser surgery (TLS). In the last decade, TLS has become an important tool in the treatment of laryngeal cancer and has become the standard approach in many institutions. The aim of this study was to review the experience of a single center institution with TLS for ear...

Objective Failure to localize the primary tumor site in head and neck carcinoma of unknown origin after imaging endoscopic evaluation leads increased treatment-related morbidity. The use transoral laser microsurgery improve detection identification is described this article. Methods A retrospective cohort 71 consecutive cases cervical an source from 2006 until 2012 a single academic institution...

Droplet-droplet interactions is ubiquitous in various applications ranging from medical diagnostics to enhancing and optimizing liquid jet propulsion. We employ an experimental technique where the laser pulse interacts with a micron-sized droplet causes optical breakdown. The synergy of nanosecond isolated spherical accurately controlled manipulated influence deformation fragmentation array dro...

We report on experiments directly in living cells that reveal the regulation of kinetochore function by tension. X and Y sex chromosomes in crane fly (Nephrotoma suturalis) spermatocytes exhibit an atypical segregation mechanism in which each univalent maintains K-fibers to both poles. During anaphase, each maintains a leading fiber (which shortens) to one pole and a trailing fiber (which elong...