Biopsy tissues from a 68-year-old Japanese man with metastases to axillary lymph nodes of a recurrent esophageal carcinoma were adapted to cell culture conditions and a continuously growing tumor cell line was developed. Immunohistochemical staining revealed that these cells contained keratinous material and the electron microscopic study revealed the presence of tonofilaments. Thus, this line,...

background and purpose: different methods such as surgery, chemotherapy, radiotherapy, hormone therapy and immunotherapy are used for treatment of melanoma cancer. unfortunately they don't always have desirable results and they may have unfavorable side effects. researchers try to find new, more effective drugs with low side effects. in this study we evaluated the cytotoxic effect of aca-1...

background: lactoferrin is a glycoprotein with a molecular weight of 80 kda, as a member of the transferring family. in recent investigations the important biological properties such as anti microbial, antiviral and anticancer activity of lactoferrin were studied. in the present study, the effect of antitumor induced by lactoferin was evaluated on stomach cancer cell ags. materials and methods:...

The objective of this study was to compare monoclonal antibody production in hollow fiber bioreactor systems and murine ascites to determine the feasibility of the bioreactor system as a potential alternative to the use of mice. Three hybridoma cell lines were grown in each of three different hollow fiber bioreactor systems and in groups of 20 mice. Mice were primed with 0.5 ml pristane intrape...