The critical concentration (CC) for ethambutol testing on the Bactec MGIT 960 M. tuberculosis susceptibility testing has been questioned in recent publications. In this study, we correlate susceptibility results from the Bactec 460, MGIT 960 and embB gene sequencing to determine if the Bactec MGIT 960 adequately detects ethambutol resistance. We discovered discrepancies between the methods that...

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this research with the aims of microbial flora determination in pasteurized milk and it’s shelf life study based on microbial and physicochemical parameters were performed within 10 days. in this study, 60 pasteurized milk samples randomly were selected and then total count of bacteria and total psychrophilic bacteria count were performed. to investigate microbial flora of pasteurized milk, col...

To decrease the time and cost of processing urine cultures, we devised a critical pathway to identify and perform antibiotic susceptibility tests on commonly isolated microbial pathogens within 6 h of growth detection. The strategy was based on eliminating expensive kits and automated procedures when not required. A pathway utilizing a statistical matrix and three rapid biochemical tests requir...

Urinary Tract Infection (UTI) is the second most common infectious disease in humans. Increasing antimicrobial resistance emphasizes the urgent need for quick and reliable diagnostic tests for evidence based antibiotic use/therapy. Increasing trend in resistance to common antibiotics used for treatment of UTI due to polymicrobial infection as opposed to monomicrobial infection makes it importan...

Polymicrobial aetiology in community-acquired pneumonia (CAP) is more common than previously recognized. This growing new entity can influence inflammation, host immunity and disease outcomes in CAP patients. However, the true incidence is complicated to determine and probably underestimated due mainly to many cases going undetected, particularly in the outpatient setting, as the diagnostic yie...

Background & Aims of the Study: Given the importance of microbial contamination in creating food-borne diseases, this study was conducted to assess level of microbial contamination of pastry creams in Hamedan, Iran. Materials and Methods: Totally, 80 samples were randomly collected from the confectioneries and analyzed for microbial contamination according to Iranian nati...

We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susc...

A new procedure for rapid detection of Salmonella in foods, based on the combination of SPRINTTM, MSRVTM and Salmonella Latex TestTM, was evaluated. SPRINTTM is a system to reduce the preenrichment and selective enrichment steps to 24 hours. MSRVTM is a semi-solid selective media for detection of motile Salmonella. Salmonella Latex TestTM is a rapid latex agglutination test for Salmonella. Usin...